RRC ID 69141
Author Shinohara H, Sakai Y, Mir TA.
Title Real-time monitoring of intracellular signal transduction in PC12 cells by two-dimensional surface plasmon resonance imager.
Journal Anal Biochem
Abstract Real-time observation of intracellular process of signal transduction is very useful for biomedical and pharmaceutical applications as well as for basic research work of cell biology. The conventional methods used to observe intracellular reactions have not been convenient with several steps such as labeling and washing steps prior to the readout. Consequently, there is a critical need for label-free observation techniques for monitoring intracellular reactions. For feasible and reagentless observation of intracellular alterations in real time, we examined the use of a high-resolution two-dimensional surface plasmon resonance (2D-SPR) imager for monitoring of intracellular signal transduction that was mainly translocation of protein kinase C via local refractive index change in PC12 cells adhered on a gold sensor slide without any indicator reagent. PC12 cells were stimulated with KCl and phorbol-12-myristate-13-acetate (PMA, a protein kinase C [PKC] activator) at different concentrations in order to induce intracellular PKC translocation. 2D-SPR signal (reflection intensity change) is very consistent with the cellular response normally detected for these stimulants. Our results suggest that complex intracellular reactions could be real-time monitored and characterized by the 2D-SPR imager. It is further expected that signal transmission that was followed by the translocation of signaling proteins could be observed at the single cell level with the high-resolution 2D-SPR imager.
Volume 441(2)
Pages 185-9
Published 2013-10-15
DOI 10.1016/j.ab.2013.07.017
PII S0003-2697(13)00335-7
PMID 23891634
MeSH Animals PC12 Cells / metabolism* Phorbol Esters / metabolism Potassium Chloride / metabolism Protein Kinase C / analysis Protein Kinase C / metabolism Protein Transport Rats Signal Transduction* Surface Plasmon Resonance / methods*
Human and Animal Cells PC-12(RCB0009)