RRC ID 69174
Author Kawai K, Takehara S, Kashio T, Morii M, Sugihara A, Yoshimura H, Ito A, Hattori M, Toda Y, Kojima M, Takebayashi Y, Furuumi H, Nonomura KI, Mikami B, Akagi T, Sakakibara H, Kitano H, Matsuoka M, Ueguchi-Tanaka M.
Title Evolutionary alterations in gene expression and enzymatic activities of gibberellin 3-oxidase 1 in Oryza.
Journal Commun Biol
Abstract Proper anther and pollen development are important for plant reproduction. The plant hormone gibberellin is important for anther development in rice, but its gametophytic functions remain largely unknown. Here, we report the functional and evolutionary analyses of rice gibberellin 3-oxidase 1 (OsGA3ox1), a gibberellin synthetic enzyme specifically expressed in the late developmental stages of anthers. Enzymatic and X-ray crystallography analyses reveal that OsGA3ox1 has a higher GA7 synthesis ratio than OsGA3ox2. In addition, we generate an osga3ox1 knockout mutant by genome editing and demonstrate the bioactive gibberellic acid synthesis by the OsGA3ox1 action during starch accumulation in pollen via invertase regulation. Furthermore, we analyze the evolution of Oryza GA3ox1s and reveal that their enzyme activity and gene expression have evolved in a way that is characteristic of the Oryza genus and contribute to their male reproduction ability.
Volume 5(1)
Pages 67
Published 2022-1-19
DOI 10.1038/s42003-022-03008-5
PII 10.1038/s42003-022-03008-5
PMID 35046494
PMC PMC8770518
MeSH Evolution, Molecular* Gene Expression Regulation, Plant* Genes, Plant Mixed Function Oxygenases / genetics* Mixed Function Oxygenases / metabolism Oryza / enzymology Oryza / genetics* Plant Proteins / genetics* Plant Proteins / metabolism
IF 4.165
Resource
Rice W0106 W1514 W1361 W1805 W1527 W1166 W0017 W0613 W0008 W0654 W0003 W1356 W1220 W0001 W1529