RRC ID |
69188
|
Author |
Sakai S, Liu Y, Mah EJ, Taya M.
|
Title |
Horseradish peroxidase/catalase-mediated cell-laden alginate-based hydrogel tube production in two-phase coaxial flow of aqueous solutions for filament-like tissues fabrication.
|
Journal |
Biofabrication
|
Abstract |
We report a method for preparing cell-laden hydrogel tubes. This method uses a coaxial double-orifice spinneret, simpler than triple-orifice spinnerets which have been used for preparing similar constructs. The intended application was to create a template for preparing filament-like structures composed of two heterogeneous living cell layers. An aqueous solution containing an alginate derivative possessing phenolic hydroxyl moieties (Alg-Ph), catalase and horseradish peroxidase (HRP) was extruded into an ambient flow of H(2)O(2) aqueous solution. This operation enabled the Alg-Ph solution to be gellable through a HRP-catalyzed reaction, cross-linking the Ph moieties together. By altering flow rates of the Alg-Ph and H(2)O(2) solutions along with the concentrations of catalase and H(2)O(2), the diameter and membrane thickness of the hydrogel tubes were controllable between 250-550 µm and 70-140 µm, respectively. The viability of the HeLa cells enclosed in the hydrogel tubes with a diameter of 300 µm and a membrane thickness of 80 µm was 95.4%. Subsequently, the enclosed HeLa cells grew and filled the hollow core. A filament-like structure of HeLa cells covered with a layer of fibroblast 10T1/2 cells was obtained when confluency of fibroblast 10T1/2 cells was reached and the hydrogel matrix was degraded with alginate lyase.
|
Volume |
5(1)
|
Pages |
015012
|
Published |
2013-3-1
|
DOI |
10.1088/1758-5082/5/1/015012
|
PMID |
23319520
|
MeSH |
Alginates / chemistry*
Biocompatible Materials / chemical synthesis*
Biocompatible Materials / chemistry
Bioengineering / instrumentation
Bioengineering / methods*
Catalase / chemistry
Cell Proliferation
Cell Survival
Fibroblasts / cytology
Glucuronic Acid / chemistry
HeLa Cells
Hexuronic Acids / chemistry
Horseradish Peroxidase / chemistry
Humans
Hydrogel, Polyethylene Glycol Dimethacrylate / chemical synthesis*
Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry
Tissue Engineering / instrumentation*
Tissue Scaffolds / chemistry*
|
IF |
8.213
|
Resource |
Human and Animal Cells |
HeLa
10T1/2(RCB0247) |