論文 - 詳細
| RRC ID | 69201 |
|---|---|
| 著者 | Oda-Ishii I, Yu D, Satou Y. |
| タイトル | Two distinct motifs for Zic-r.a drive specific gene expression in two cell lineages. |
| ジャーナル | Development |
| Abstract |
Zic-r.a, a maternal transcription factor, specifies posterior fate in ascidian embryos. However, its direct target, Tbx6-r.b, does not contain typical Zic-r.a-binding sites in its regulatory region. Using an in vitro selection assay, we found that Zic-r.a binds to sites dissimilar to the canonical motif, by which it activates Tbx6-r.b in a sub-lineage of muscle cells. These sites with non-canonical motifs have weak affinity for Zic-r.a; therefore, it activates Tbx6-r.b only in cells expressing Zic-r.a abundantly. Meanwhile, we found that Zic-r.a expressed zygotically in late embryos activates neural genes through canonical sites. Because different zinc-finger domains of Zic-r.a are important for driving reporters with canonical and non-canonical sites, it is likely that the non-canonical motif is not a divergent version of the canonical motif. In other words, our data indicate that the non-canonical motif represents a motif distinct from the canonical motif. Thus, Zic-r.a recognizes two distinct motifs to activate two sets of genes at two timepoints in development. This article has an associated 'The people behind the papers' interview. |
| 巻・号 | 148(11) |
| 公開日 | 2021-6-1 |
| DOI | 10.1242/dev.199538 |
| PII | 269043 |
| PMID | 34100063 |
| MeSH | Animals Binding Sites Cell Lineage / genetics* Cell Lineage / physiology* Ciona intestinalis / genetics Embryo, Nonmammalian / metabolism Gene Expression* Gene Expression Regulation, Developmental T-Box Domain Proteins / metabolism Transcription Factors / metabolism Urochordata / embryology Urochordata / genetics Zinc Fingers / genetics* |
| IF | 5.611 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| カタユウレイボヤ・(ニッポンウミシダ) | Wild C. int |