| RRC ID |
69211
|
| Author |
Kiboku T, Katoh T, Nakamura A, Kitamura A, Kinjo M, Murakami Y, Takahashi M.
|
| Title |
Nonmuscle myosin II folds into a 10S form via two portions of tail for dynamic subcellular localization.
|
| Journal |
Genes Cells
|
| Abstract |
Nonmuscle myosin II forms a folded conformation (10S form) in the inactivated state; however, the physiological importance of the 10S form is still unclear. To investigate the role of 10S form, we generated a chimeric mutant of nonmuscle myosin IIB (IIB-SK1·2), in which S1462-R1490 and L1551-E1577 were replaced with the corresponding portions of skeletal muscle myosin heavy chain. The IIB-SK1·2 mutant did not fold into a 10S form under physiological condition in vitro. IIB-SK1·2 was less dynamic by stabilizing the filamentous form and accumulated in the posterior region of migrating cells. IIB-SK1·2 functioned properly in cytokinesis but altered migratory properties; the rate and directional persistence were increased by IIB-SK1·2 expression. Surprisingly, endogenous nonmuscle myosin IIA was excluded from the posterior region of migrating cells expressing IIB-SK1·2, which may underlie the change of the cellular migratory properties. These results suggest that the 10S form is necessary for maintaining nonmuscle myosin II in an unassembled state and for recruitment of nonmuscle myosin II to a specific region of the cell.
|
| Volume |
18(2)
|
| Pages |
90-109
|
| Published |
2013-2-1
|
| DOI |
10.1111/gtc.12021
|
| PMID |
23237600
|
| MeSH |
Cell Line
Cytokinesis / physiology
Humans
Intracellular Space / metabolism*
Myosin Type II / chemistry
Myosin Type II / metabolism
Nonmuscle Myosin Type IIA / chemistry
Nonmuscle Myosin Type IIA / genetics
Nonmuscle Myosin Type IIA / metabolism
Nonmuscle Myosin Type IIB / chemistry*
Nonmuscle Myosin Type IIB / genetics
Nonmuscle Myosin Type IIB / metabolism*
Protein Conformation
Protein Folding*
Protein Stability
Protein Transport
Subcellular Fractions
|
| IF |
1.655
|
| Resource |
| Human and Animal Cells |
MRC-5 SV1 TG1(RCB0207) |
