論文 - 詳細
| RRC ID | 69231 |
|---|---|
| 著者 | Burroughs AM, Kawano M, Ando Y, Daub CO, Hayashizaki Y. |
| タイトル | pre-miRNA profiles obtained through application of locked nucleic acids and deep sequencing reveals complex 5'/3' arm variation including concomitant cleavage and polyuridylation patterns. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Recent research hints at an underappreciated complexity in pre-miRNA processing and regulation. Global profiling of pre-miRNA and its potential to increase understanding of the pre-miRNA landscape is impeded by overlap with highly expressed classes of other non coding (nc) RNA. Here, we present a data set excluding these RNA before sequencing through locked nucleic acids (LNA), greatly increasing pre-miRNA sequence counts with no discernable effect on pre-miRNA or mature miRNA sequencing. Analysis of profiles generated in total, nuclear and cytoplasmic cell fractions reveals that pre-miRNAs are subject to a wide range of regulatory processes involving loci-specific 3'- and 5'-end variation entailing complex cleavage patterns with co-occurring polyuridylation. Additionally, examination of nuclear-enriched flanking sequences of pre-miRNA, particularly those derived from polycistronic miRNA transcripts, provides insight into miRNA and miRNA-offset (moRNA) production, specifically identifying novel classes of RNA potentially functioning as moRNA precursors. Our findings point to particularly intricate regulation of the let-7 family in many ways reminiscent of DICER1-independent, pre-mir-451-like processing, introduce novel and unify known forms of pre-miRNA regulation and processing, and shed new light on overlooked products of miRNA processing pathways. |
| 巻・号 | 40(4) |
| ページ | 1424-37 |
| 公開日 | 2012-2-1 |
| DOI | 10.1093/nar/gkr903 |
| PII | gkr903 |
| PMID | 22058130 |
| PMC | PMC3287202 |
| MeSH | HeLa Cells High-Throughput Nucleotide Sequencing Humans MicroRNAs / chemistry* MicroRNAs / metabolism Nucleotide Motifs Oligodeoxyribonucleotides / chemistry Oligonucleotides / chemistry* Poly U / analysis* RNA Precursors / chemistry* RNA Precursors / metabolism RNA Processing, Post-Transcriptional* RNA-Binding Proteins / metabolism Sequence Analysis, RNA Uridine / analysis Uridine / metabolism |
| IF | 11.502 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |