| RRC ID |
69248
|
| 著者 |
Dehne T, Adam X, Materne EM, Reimann MC, Krüger JP, Van Linthout S, Tschöpe C, Haag M, Sittinger M, Ringe J.
|
| タイトル |
A P19 and P19CL6 cell-based complementary approach to determine paracrine effects in cardiac tissue engineering.
|
| ジャーナル |
Cells Tissues Organs
|
| Abstract |
The negligible self-repair potential of the myocardium has led to cell-based tissue engineering approaches to restore heart function. There is more and more consensus that, in addition to cell development, paracrine effects in particular play a pivotal role in the repair of heart tissue. Here, we present two complementary murine P19 and P19CL6 embryonic carcinoma cell-based in vitro test approaches to study the potential of repair cells and the factors secreted by these cells to induce cardiomyogenesis. P19 cells were 3-dimensionally cultured in hanging drops and P19CL6 cells in a monolayer. Both systems, capable of inducible differentiation towards the cardiomyogenic lineage shown by the appearance of beating cells, the expression of connexin 43 and cardiac troponins T and I, were used to test the cardiomyogenesis-inducing potential of human cardiac-derived adherent proliferating (CardAP) cells, which are candidates for heart repair. CardAP cells in coculture as well as CardAP cell-conditioned medium initiated beating in P19 cells, depending on the cell composition and concentration of the medium. CardAP cell-dependent beating was not observed in P19CL6 cultures, but connexin 43 and cardiac troponin formation as well as expression of GATA-binding protein 4 indicated the dose-dependent stimulatory cardiomyogenic effect of human CardAP cells. In summary, in different ways, P19 and P19CL6 cells have shown their capability to detect paracrine effects of human CardAP cells. In a complementary approach, they could be beneficial for determining the stimulatory cardiomyogenic potential of candidate cardiac-repair cells in vitro.
|
| 巻・号 |
199(1)
|
| ページ |
24-36
|
| 公開日 |
2014-1-1
|
| DOI |
10.1159/000362540
|
| PII |
000362540
|
| PMID |
25170977
|
| MeSH |
Animals
Carcinoma, Embryonal
Cell Differentiation / physiology
Cell Line, Tumor
Coculture Techniques
Culture Media, Conditioned
Heart / physiology*
Humans
Mice
Myocardium / cytology
Myocardium / metabolism
Myocytes, Cardiac / cytology*
Myocytes, Cardiac / metabolism
Tissue Engineering / methods*
|
| IF |
2.064
|
| リソース情報 |
| ヒト・動物細胞 |
P19.CL6(RCB2318) |
