論文 - 詳細
| RRC ID | 69253 |
|---|---|
| 著者 | Morimoto-Kamata R, Mizoguchi S, Ichisugi T, Yui S. |
| タイトル | Cathepsin G induces cell aggregation of human breast cancer MCF-7 cells via a 2-step mechanism: catalytic site-independent binding to the cell surface and enzymatic activity-dependent induction of the cell aggregation. |
| ジャーナル | Mediators Inflamm |
| Abstract |
Neutrophils often invade various tumor tissues and affect tumor progression and metastasis. Cathepsin G (CG) is a serine protease secreted from activated neutrophils. Previously, we have shown that CG induces the formation of E-cadherin-mediated multicellular spheroids of human breast cancer MCF-7 cells; however, the molecular mechanisms involved in this process are unknown. In this study, we investigated whether CG required its enzymatic activity to induce MCF-7 cell aggregation. The cell aggregation-inducing activity of CG was inhibited by pretreatment of CG with the serine protease inhibitors chymostatin and phenylmethylsulfonyl fluoride. In addition, an enzymatically inactive S195G (chymotrypsinogen numbering) CG did not induce cell aggregation. Furthermore, CG specifically bound to the cell surface of MCF-7 cells via a catalytic site-independent mechanism because the binding was not affected by pretreatment of CG with serine protease inhibitors, and cell surface binding was also detected with S195G CG. Therefore, we propose that the CG-induced aggregation of MCF-7 cells occurs via a 2-step process, in which CG binds to the cell surface, independently of its catalytic site, and then induces cell aggregation, which is dependent on its enzymatic activity. |
| 巻・号 | 2012 |
| ページ | 456462 |
| 公開日 | 2012-1-1 |
| DOI | 10.1155/2012/456462 |
| PMID | 22919124 |
| PMC | PMC3418687 |
| MeSH | Catalytic Domain Cathepsin G / antagonists & inhibitors Cathepsin G / metabolism* Cathepsin G / pharmacology* Cell Adhesion / drug effects Cell Aggregation / drug effects* Cell Line, Tumor Humans Oligopeptides / pharmacology Phenylmethylsulfonyl Fluoride / pharmacology Protein Binding |
| IF | 3.758 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RBL 2H3(RCB2782) |