| RRC ID |
69328
|
| 著者 |
Yasuhiro Yamasaki, Ryunosuke Hirayama, Akiho Yamamoto, Koki Yuasa, Tomoyuki Shikata
|
| タイトル |
Effects of micronutrients on the detection of extracellular superoxide produced by the harmful raphidophyte Chattonella antiqua in culture
|
| ジャーナル |
Journal of Plankton Research
|
| Abstract |
Noxious Chattonella species cause many red tides and fish kills. The detection of extracellular O2− produced by these algae will be critical for the development of a rapid toxicity-evaluation method; however, such a development has been prevented by the mismatch of the extracellular O2− detection level between field and laboratory experiments. The aim of this study was to identify impediments to detect extracellular O2− in laboratory experiments. Our results indicate that Mn and Co in the medium decreased the detection levels of extracellular O2− produced by Chattonella antiqua, and the effect by Mn was stronger than that by Co. IC50 of the radical-scavenging activity of Mn was 17.5 μM, and little activity was detected at 8.75 μM or less. Furthermore, the level of extracellular O2− production by C. antiqua in Mn-depleted medium varied greatly under various culture conditions; the extracellular O2− production level of C. antiqua was high under conditions similar to those under which blooms develop in the field. Therefore, Mn is a key scavenger of extracellular O2−, and amounts of Mn must be carefully considered whenever extracellular O2− production levels of phytoplankton cultured with other kinds of media are measured.
|
| 巻・号 |
44
|
| ページ |
36-47
|
| 公開日 |
2022-1-25
|
| DOI |
10.1093/plankt/fbab087
|
| IF |
2.149
|
| リソース情報 |
| 藻類 |
NIES-1 |
