RRC ID 69339
Author Yoshino K, Motoyama S, Koyota S, Shibuya K, Sato Y, Sasaki T, Wakita A, Saito H, Minamiya Y, Sugiyama T, Ogawa J.
Title Identification of insulin-like growth factor 2 mRNA-binding protein 3 as a radioresistance factor in squamous esophageal cancer cells.
Journal Dis Esophagus
Abstract Identification of reliable markers of radiosensitivity and the key molecules that donate susceptibility to anticancer treatments to esophageal cancer cells would be highly desirable. We found that the mRNA expression of insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) was higher in radioresistant TE-5 and TE-9 cells than in radiosensitive TE-12 cloneA1 cells. Conversely, knocking down expression of IGF2BP3 mRNA in TE-5 and TE-9 cells using small interfering RNA significantly enhanced their radiosensitivity. Furthermore, patients with squamous cell esophageal cancers strongly expressing IGF2BP3 tended to respond poorly to chemoradiation. These data suggest that IGF2BP3 may be a key marker of radiosensitivity that diminishes the susceptibility of squamous cell esophageal cancer cells to radiotherapy. IGF2BP3 may, thus, be a useful target for improving radiotherapy for patients with esophageal squamous cell carcinoma.
Volume 27(5)
Pages 479-84
Published 2014-7-1
DOI 10.1111/j.1442-2050.2012.01415.x
PMID 22989274
MeSH Carcinoma, Squamous Cell / metabolism* Carcinoma, Squamous Cell / pathology Carcinoma, Squamous Cell / radiotherapy Cell Line, Tumor Chemoradiotherapy Esophageal Neoplasms / metabolism* Esophageal Neoplasms / pathology Esophageal Neoplasms / radiotherapy Gene Knockdown Techniques Humans RNA, Messenger / metabolism RNA, Small Interfering RNA-Binding Proteins / genetics* RNA-Binding Proteins / metabolism Radiation Tolerance / genetics*
IF 2.386
Human and Animal Cells TE-5(RCB1949)