RRC ID 69415
Author Nagasaki A, Inotsume K, Kanada M, Uyeda TQ.
Title Phospholipase D is essential for keratocyte-like migration of NBT-II cells.
Journal Cell Struct Funct
Abstract NBT-II cells on collagen-coated substrates move rapidly and persistently, maintaining a semi-circular shape with a large lamellipodium, in a manner similar to fish keratocytes. The inhibitor of phospholipase D (PLD), n-butanol, completely blocked the migration and disturbed the characteristic localization of actin along the edge of lamellipodia. To investigate the functional difference between the two isozymes of PLD (PLD1 and PLD2), we transfected NBT-II cells with vectors expressing shRNA to deplete PLD1 or PLD2. Depletion of both PLD1 and 2 by RNA interference reduced the velocity of the migration, but depletion of PLD2 inhibited motility more severely than that of PLD1. Furthermore, GFP-PLD2 was localized to the protruding regions of lamellipodia in migrating cells. Thus, PLD is essential for the maintenance of keratocyte-like locomotion of NBT-II cells, presumably by regulating the actin cytoskeleton.
Volume 33(1)
Pages 27-33
Published 2008-1-1
DOI 10.1247/csf.07042
PII JST.JSTAGE/csf/07042
PMID 18344600
MeSH 1-Butanol / pharmacology Actin Cytoskeleton / metabolism* Animals Cell Line Cell Line, Tumor Cell Movement / drug effects Cell Movement / physiology* DNA, Complementary / metabolism Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Humans Phospholipase D / genetics Phospholipase D / metabolism* Pseudopodia / metabolism RNA Interference Rats Transfection
IF 2.429
Human and Animal Cells NBT-T1(RCB1369) NBT-T2(RCB1370)