論文 - 詳細
| RRC ID | 69459 |
|---|---|
| 著者 | Kono Y, Kawakami S, Higuchi Y, Maruyama K, Yamashita F, Hashida M. |
| タイトル | Tumour-associated macrophages targeted transfection with NF-κB decoy/mannose-modified bubble lipoplexes inhibits tumour growth in tumour-bearing mice. |
| ジャーナル | J Drug Target |
| Abstract |
Tumour-associated macrophages (TAM) exhibit an M2 phenotype that promotes tumour progression, and conversion of M2 TAM toward a tumouricidal M1 phenotype is a promising anti-cancer therapy. As NF-κB is a key regulator of macrophage polarization, we developed an in vivo TAM-targeting delivery system that combines mannose-modified bubble liposomes/NF-κB decoy complexes (Man-PEG bubble lipoplexes) and ultrasound (US) exposure. We investigated the effects of NF-κB decoy transfection on TAM phenotype in solid tumour-bearing mice. Post-transfection tumour growth and survival rates were also recorded. Th2 cytokine (IL-10) level in TAM was significantly lower by NF-κB decoy transfection using Man-PEG bubble lipoplexes and US exposure, while Th1 cytokine levels (IL-1β, TNF-α and IL-6) were significantly higher when compared with controls. In addition, mRNA levels of vascular endothelial growth factor, matrix metalloproteinase-9 and arginase were significantly lower in TAM post-NF-κB decoy transfection. Importantly, TAM-targeted NF-κB decoy transfection inhibited tumour growth and prolonged survival rates in mice. Therefore, TAM-targeted NF-κB decoy transfection using Man-PEG bubble lipoplexes and US exposure may be an effective approach for anti-cancer therapy based on TAM phenotypic conversion from M2 toward M1. |
| 巻・号 | 22(5) |
| ページ | 439-49 |
| 公開日 | 2014-6-1 |
| DOI | 10.3109/1061186X.2014.880443 |
| PMID | 24579693 |
| MeSH | Animals Colonic Neoplasms / genetics Colonic Neoplasms / immunology Colonic Neoplasms / pathology Colonic Neoplasms / therapy* Drug Carriers / chemistry* Female Gene Transfer Techniques Liposomes Macrophages / metabolism* Mannose / chemistry* Mice, Inbred BALB C Neoplasm Transplantation Oligodeoxyribonucleotides / genetics* Polyethylene Glycols / chemistry Real-Time Polymerase Chain Reaction Survival Analysis Transfection Ultrasonics |
| IF | 3.38 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | Colon-26(RCB2657) |