| RRC ID |
69460
|
| 著者 |
Islam W, Matsumoto Y, Fang J, Harada A, Niidome T, Ono K, Tsutsuki H, Sawa T, Imamura T, Sakurai K, Fukumitsu N, Yamamoto H, Maeda H.
|
| タイトル |
Polymer-conjugated glucosamine complexed with boric acid shows tumor-selective accumulation and simultaneous inhibition of glycolysis.
|
| ジャーナル |
Biomaterials
|
| Abstract |
We synthesized unique water-soluble synthetic-polymer, styrene-maleic acid copolymer (SMA) conjugated glucosamine (SG); which formed a stable complex with boric acid (BA). This complex had a mean particle size of 15 nm by light scattering, and single peak in gel permeation chromatography. The particles were taken up by tumor cells five times faster than free BA in vitro and liberated BA at acidic tumor pH (5-7). Liberated BA inhibited glycolysis and resulted in tumor suppression in vivo. Intravenously injected SGB-complex did bind with albumin, and plasma half-life was about 8 h in mice, and accumulated to tumor tissues about 10 times more than in normal organs. IC50 of SGB-complex for HeLa cells under pO2 of 6-9% was about 20 μg/ml (free BA equivalent), 150 times more potent than free BA. Neutron irradiation of human oral cancer cells with SGB-complex resulted in 16 times greater cell-killing than that without SGB-complex. In vivo antitumor effect was evaluated after neutron irradiation only once in SCC VII tumor bearing mice and significant tumor suppression was confirmed. These results indicate that SGB-complex is a unique multifunctional anticancer agent with much more potent activity under low pO2 conditions as in large advanced cancers.
|
| 巻・号 |
269
|
| ページ |
120631
|
| 公開日 |
2021-2-1
|
| DOI |
10.1016/j.biomaterials.2020.120631
|
| PII |
S0142-9612(20)30878-4
|
| PMID |
33450582
|
| MeSH |
Animals
Boric Acids
Cell Line, Tumor
Glucosamine*
Glycolysis
HeLa Cells
Humans
Mice
Polymers*
|
| IF |
10.317
|
| リソース情報 |
| ヒト・動物細胞 |
SAS(RCB1974) |
