| RRC ID |
69587
|
| Author |
Azuma R, Yamasaki T, Sano K, Munekane M, Matsuoka Y, Yamada KI, Mukai T.
|
| Title |
A radioiodinated nitroxide probe with improved stability against bioreduction for in vivo detection of lipid radicals.
|
| Journal |
Free Radic Biol Med
|
| Abstract |
It is well known that lipid carbon radicals (lipid radicals) are the origin of lipid peroxidation and are involved in various diseases such as cancer. Therefore, the in vivo detection of lipid radicals would be expected to lead to early diagnosis of these diseases. However, there are no methods for measuring lipid radicals in vivo. Nitroxides are known to be highly reactive with lipid radicals, but they tend to be reduced in vivo. Focusing on the excellent detection sensitivity of nuclear medical imaging, we have developed a radioiodinated nitroxide derivative with resistance to bioreduction for the in vivo detection of lipid radicals. The desired compound was obtained successfully and was highly stable against bioreduction while maintaining high reactivity toward lipid radicals. The I-125 labeling was efficacious with radiochemical yields of 84-87% and radiochemical purities of >99%. A cellular uptake assay showed that the radioiodinated compound was significantly taken up by cells under lipid radical-producing conditions compared to that in the absence of lipid radical production. A biodistribution study indicated that the radioiodinated compound accumulated more in organs where lipid peroxidation was promoted than the methoxyamine derivative, which lost reactivity to lipid radicals. These results indicated that the developed probe became trapped in cells or organs by reacting with lipid radicals. Thus, the radioiodinated nitroxide is a candidate probe for in vivo detection of lipid radicals.
|
| Volume |
163
|
| Pages |
297-305
|
| Published |
2021-2-1
|
| DOI |
10.1016/j.freeradbiomed.2020.12.028
|
| PII |
S0891-5849(20)31691-9
|
| PMID |
33359688
|
| MeSH |
Antioxidants*
Cyclic N-Oxides
Electron Spin Resonance Spectroscopy
Free Radicals
Iodine Radioisotopes*
Lipids
Nitrogen Oxides
Tissue Distribution
|
| IF |
6.17
|
| Resource |
| Human and Animal Cells |
Hep G2(RCB1648) |
