論文 - 詳細
| RRC ID | 69605 |
|---|---|
| 著者 | Ishida T, Obata Y, Ohara N, Matsushita H, Sato S, Uenaka A, Saika T, Miyamura T, Chayama K, Nakamura Y, Wada H, Yamashita T, Morishima T, Old LJ, Nakayama E. |
| タイトル | Identification of the HERV-K gag antigen in prostate cancer by SEREX using autologous patient serum and its immunogenicity. |
| ジャーナル | Cancer Immun |
| Abstract |
The prostate cancer HERV-K gag-related NGO-Pr-54 antigen was identified by SEREX analysis using autologous patient serum. NGO-Pr-54 mRNA was observed to be faintly expressed in normal prostate and strongly expressed in a variety of cancers, including ovarian cancer (5/8), prostate cancer (6/9), and leukemia (5/14). A phage plaque assay showed that a strong reaction was constantly observed with clone ZH042 in which the 5' end of NGO-Pr-54 is deleted, suggesting that it contained the sequence coding for the protein product. A TI-35 mAb was produced using a recombinant protein (438 aa) deduced from the sequence of ZH042. Transfection of clone ZH042 into 293T cells resulted in the production of an approximately 50-kDa molecule visualized by Western blotting. Natural production of the molecule was confirmed in a SK-MEL-23 melanoma cell line. An indirect immunofluorescence assay showed that NGO-Pr-54 protein was expressed on the cell surface as well as in the cytoplasm. Cell surface expression was confirmed by flow cytometry using the TI-35 mAb. The antibody response against NGO-Pr-54 was observed in patients with bladder (5.1%), liver (4.1%), lung (3.4%), ovarian (5.6%), and prostate (4.2%) cancer, as well as with malignant melanoma (13.2%). |
| 巻・号 | 8 |
| ページ | 15 |
| 公開日 | 2008-11-13 |
| PII | 081042 |
| PMID | 19006261 |
| PMC | PMC2935773 |
| MeSH | Aged, 80 and over Amino Acid Sequence Animals Antibodies, Monoclonal Antigens, Neoplasm / genetics* Antigens, Neoplasm / immunology COS Cells Chlorocebus aethiops Cloning, Molecular Endogenous Retroviruses Female Fluorescent Antibody Technique, Indirect Gene Expression Regulation, Neoplastic Gene Products, gag* Humans Immune Sera / analysis Leukemia / genetics Leukemia / immunology Male Melanoma / genetics Melanoma / immunology Mice Mice, Inbred BALB C Molecular Sequence Data Neoplasm Proteins / genetics* Ovarian Neoplasms / genetics Ovarian Neoplasms / immunology Prostate / immunology Prostate / metabolism Prostatic Neoplasms / genetics* Prostatic Neoplasms / immunology Recombinant Proteins / genetics Transfection |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ACC-MESO-1(RCB2292) ACC-MESO-4(RCB2293) |