RRC ID 69605
Author Ishida T, Obata Y, Ohara N, Matsushita H, Sato S, Uenaka A, Saika T, Miyamura T, Chayama K, Nakamura Y, Wada H, Yamashita T, Morishima T, Old LJ, Nakayama E.
Title Identification of the HERV-K gag antigen in prostate cancer by SEREX using autologous patient serum and its immunogenicity.
Journal Cancer Immun
Abstract The prostate cancer HERV-K gag-related NGO-Pr-54 antigen was identified by SEREX analysis using autologous patient serum. NGO-Pr-54 mRNA was observed to be faintly expressed in normal prostate and strongly expressed in a variety of cancers, including ovarian cancer (5/8), prostate cancer (6/9), and leukemia (5/14). A phage plaque assay showed that a strong reaction was constantly observed with clone ZH042 in which the 5' end of NGO-Pr-54 is deleted, suggesting that it contained the sequence coding for the protein product. A TI-35 mAb was produced using a recombinant protein (438 aa) deduced from the sequence of ZH042. Transfection of clone ZH042 into 293T cells resulted in the production of an approximately 50-kDa molecule visualized by Western blotting. Natural production of the molecule was confirmed in a SK-MEL-23 melanoma cell line. An indirect immunofluorescence assay showed that NGO-Pr-54 protein was expressed on the cell surface as well as in the cytoplasm. Cell surface expression was confirmed by flow cytometry using the TI-35 mAb. The antibody response against NGO-Pr-54 was observed in patients with bladder (5.1%), liver (4.1%), lung (3.4%), ovarian (5.6%), and prostate (4.2%) cancer, as well as with malignant melanoma (13.2%).
Volume 8
Pages 15
Published 2008-11-13
PII 081042
PMID 19006261
PMC PMC2935773
MeSH Aged, 80 and over Amino Acid Sequence Animals Antibodies, Monoclonal Antigens, Neoplasm / genetics* Antigens, Neoplasm / immunology COS Cells Chlorocebus aethiops Cloning, Molecular Endogenous Retroviruses Female Fluorescent Antibody Technique, Indirect Gene Expression Regulation, Neoplastic Gene Products, gag* Humans Immune Sera / analysis Leukemia / genetics Leukemia / immunology Male Melanoma / genetics Melanoma / immunology Mice Mice, Inbred BALB C Molecular Sequence Data Neoplasm Proteins / genetics* Ovarian Neoplasms / genetics Ovarian Neoplasms / immunology Prostate / immunology Prostate / metabolism Prostatic Neoplasms / genetics* Prostatic Neoplasms / immunology Recombinant Proteins / genetics Transfection
Human and Animal Cells ACC-MESO-1(RCB2292) ACC-MESO-4(RCB2293)