| RRC ID |
69605
|
| 著者 |
Ishida T, Obata Y, Ohara N, Matsushita H, Sato S, Uenaka A, Saika T, Miyamura T, Chayama K, Nakamura Y, Wada H, Yamashita T, Morishima T, Old LJ, Nakayama E.
|
| タイトル |
Identification of the HERV-K gag antigen in prostate cancer by SEREX using autologous patient serum and its immunogenicity.
|
| ジャーナル |
Cancer Immun
|
| Abstract |
The prostate cancer HERV-K gag-related NGO-Pr-54 antigen was identified by SEREX analysis using autologous patient serum. NGO-Pr-54 mRNA was observed to be faintly expressed in normal prostate and strongly expressed in a variety of cancers, including ovarian cancer (5/8), prostate cancer (6/9), and leukemia (5/14). A phage plaque assay showed that a strong reaction was constantly observed with clone ZH042 in which the 5' end of NGO-Pr-54 is deleted, suggesting that it contained the sequence coding for the protein product. A TI-35 mAb was produced using a recombinant protein (438 aa) deduced from the sequence of ZH042. Transfection of clone ZH042 into 293T cells resulted in the production of an approximately 50-kDa molecule visualized by Western blotting. Natural production of the molecule was confirmed in a SK-MEL-23 melanoma cell line. An indirect immunofluorescence assay showed that NGO-Pr-54 protein was expressed on the cell surface as well as in the cytoplasm. Cell surface expression was confirmed by flow cytometry using the TI-35 mAb. The antibody response against NGO-Pr-54 was observed in patients with bladder (5.1%), liver (4.1%), lung (3.4%), ovarian (5.6%), and prostate (4.2%) cancer, as well as with malignant melanoma (13.2%).
|
| 巻・号 |
8
|
| ページ |
15
|
| 公開日 |
2008-11-13
|
| PII |
081042
|
| PMID |
19006261
|
| PMC |
PMC2935773
|
| MeSH |
Aged, 80 and over
Amino Acid Sequence
Animals
Antibodies, Monoclonal
Antigens, Neoplasm / genetics*
Antigens, Neoplasm / immunology
COS Cells
Chlorocebus aethiops
Cloning, Molecular
Endogenous Retroviruses
Female
Fluorescent Antibody Technique, Indirect
Gene Expression Regulation, Neoplastic
Gene Products, gag*
Humans
Immune Sera / analysis
Leukemia / genetics
Leukemia / immunology
Male
Melanoma / genetics
Melanoma / immunology
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Neoplasm Proteins / genetics*
Ovarian Neoplasms / genetics
Ovarian Neoplasms / immunology
Prostate / immunology
Prostate / metabolism
Prostatic Neoplasms / genetics*
Prostatic Neoplasms / immunology
Recombinant Proteins / genetics
Transfection
|
| リソース情報 |
| ヒト・動物細胞 |
ACC-MESO-1(RCB2292)
ACC-MESO-4(RCB2293) |
