論文 - 詳細
| RRC ID | 69634 |
|---|---|
| 著者 | Sakamoto M, Inoue M, Takeuchi A, Kobari S, Yokoyama T, Horigane SI, Takemoto-Kimura S, Abe M, Sakimura K, Kano M, Kitamura K, Fujii H, Bito H. |
| タイトル | A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo. |
| ジャーナル | Cell Rep Methods |
| Abstract |
Genetically encoded calcium indicators (GECIs) are widely used to measure calcium transients in neuronal somata and processes, and their use enables the determination of action potential temporal series in a large population of neurons. Here, we generate a transgenic mouse line expressing a highly sensitive green GECI, G-CaMP9a, in a Flp-dependent manner in excitatory and inhibitory neuronal subpopulations downstream of a strong CAG promoter. Combining this reporter mouse with viral or mouse genetic Flp delivery methods produces a robust and stable G-CaMP9a expression in defined neuronal populations without detectable detrimental effects. In vivo two-photon imaging reveals spontaneous and sensory-evoked calcium transients in excitatory and inhibitory ensembles with cellular resolution. Our results show that this reporter line allows long-term, cell-type-specific investigation of neuronal activity with enhanced resolution in defined populations and facilitates dissecting complex dynamics of neural networks in vivo. |
| 巻・号 | 2(2) |
| ページ | 100168 |
| 公開日 | 2022-2-28 |
| DOI | 10.1016/j.crmeth.2022.100168 |
| PII | S2667-2375(22)00020-0 |
| PMID | 35474964 |
| PMC | PMC9017135 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 26 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pBS-CaMKIIpro-G-CaMP9a-WPRE (RDB19172) |