RRC ID 69634
Author Sakamoto M, Inoue M, Takeuchi A, Kobari S, Yokoyama T, Horigane SI, Takemoto-Kimura S, Abe M, Sakimura K, Kano M, Kitamura K, Fujii H, Bito H.
Title A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo.
Journal Cell Rep Methods
Abstract Genetically encoded calcium indicators (GECIs) are widely used to measure calcium transients in neuronal somata and processes, and their use enables the determination of action potential temporal series in a large population of neurons. Here, we generate a transgenic mouse line expressing a highly sensitive green GECI, G-CaMP9a, in a Flp-dependent manner in excitatory and inhibitory neuronal subpopulations downstream of a strong CAG promoter. Combining this reporter mouse with viral or mouse genetic Flp delivery methods produces a robust and stable G-CaMP9a expression in defined neuronal populations without detectable detrimental effects. In vivo two-photon imaging reveals spontaneous and sensory-evoked calcium transients in excitatory and inhibitory ensembles with cellular resolution. Our results show that this reporter line allows long-term, cell-type-specific investigation of neuronal activity with enhanced resolution in defined populations and facilitates dissecting complex dynamics of neural networks in vivo.
Volume 2(2)
Pages 100168
Published 2022-2-28
DOI 10.1016/j.crmeth.2022.100168
PII S2667-2375(22)00020-0
PMID 35474964
PMC PMC9017135
DNA material pBS-CaMKIIpro-G-CaMP9a-WPRE (RDB19172)