| RRC ID |
69634
|
| Author |
Sakamoto M, Inoue M, Takeuchi A, Kobari S, Yokoyama T, Horigane SI, Takemoto-Kimura S, Abe M, Sakimura K, Kano M, Kitamura K, Fujii H, Bito H.
|
| Title |
A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo.
|
| Journal |
Cell Rep Methods
|
| Abstract |
Genetically encoded calcium indicators (GECIs) are widely used to measure calcium transients in neuronal somata and processes, and their use enables the determination of action potential temporal series in a large population of neurons. Here, we generate a transgenic mouse line expressing a highly sensitive green GECI, G-CaMP9a, in a Flp-dependent manner in excitatory and inhibitory neuronal subpopulations downstream of a strong CAG promoter. Combining this reporter mouse with viral or mouse genetic Flp delivery methods produces a robust and stable G-CaMP9a expression in defined neuronal populations without detectable detrimental effects. In vivo two-photon imaging reveals spontaneous and sensory-evoked calcium transients in excitatory and inhibitory ensembles with cellular resolution. Our results show that this reporter line allows long-term, cell-type-specific investigation of neuronal activity with enhanced resolution in defined populations and facilitates dissecting complex dynamics of neural networks in vivo.
|
| Volume |
2(2)
|
| Pages |
100168
|
| Published |
2022-2-28
|
| DOI |
10.1016/j.crmeth.2022.100168
|
| PII |
S2667-2375(22)00020-0
|
| PMID |
35474964
|
| PMC |
PMC9017135
|
| Resource |
| DNA material |
pBS-CaMKIIpro-G-CaMP9a-WPRE (RDB19172) |
