| RRC ID |
69679
|
| 著者 |
Feng X, Lu J, Wu Y, Xu H.
|
| タイトル |
MiR-18a-3p improves cartilage matrix remodeling and inhibits inflammation in osteoarthritis by suppressing PDP1.
|
| ジャーナル |
J Physiol Sci
|
| Abstract |
Osteoarthritis (OA) is a degenerative disease characterized by synovial inflammation. MiR-18a-3p was reported to be downregulated in knee anterior cruciate ligament of OA patients. In the present study, the specific functions and mechanism of miR-18a-3p in OA were explored. An in vitro model of OA was established using 10 ng/ml IL-1β to treat ATDC5 cells, and medial meniscus instability surgery was performed on Wistar rats to establish in vivo rat model of OA. RT-qPCR revealed that miR-18a-3p was downregulated in IL-1β-stimulated ATDC5 cells. MiR-18a-3p overexpression inhibited secretion of inflammatory cytokines and concentration of matrix metalloproteinases, as shown by ELISA and western blotting. The binding relation between miR-18a-3p and pyruvate dehydrogenase phosphatase catalytic subunit 1 (PDP1) was detected by luciferase reporter assays. MiR-18a-3p targeted PDP1 and negatively regulated PDP1 expression. Results of rescue assays revealed that PDP1 upregulation reserved the suppressive effect of miR-18a-3p overexpression on levels of inflammatory cytokines and matrix metalloproteinases in IL-1β-stimulated ATDC5 cells. H&E staining was used to observe pathological changes of synovial tissues in the knee joint of Wistar rats. Safranin O-fast green/hematoxylin was used to stain cartilage samples of knee joints. MiR-18a-3p overexpression suppressed OA progression in vivo. Overall, miR-18a-3p improves cartilage matrix remodeling and suppresses inflammation in OA by targeting PDP1.
|
| 巻・号 |
72(1)
|
| ページ |
3
|
| 公開日 |
2022-2-11
|
| DOI |
10.1186/s12576-022-00827-3
|
| PII |
10.1186/s12576-022-00827-3
|
| PMID |
35148687
|
| MeSH |
Animals
Apoptosis
Cartilage
Chondrocytes
Humans
Inflammation / genetics
Interleukin-1beta
MicroRNAs* / genetics
Osteoarthritis* / genetics
Protein Phosphatase 2C*
Rats
Rats, Wistar
Signal Transduction
|
| IF |
2.804
|
| リソース情報 |
| ヒト・動物細胞 |
ATDC5(RCB0565) |
