RRC ID 69784
Author Matsuura S, Takayama T, Oyama TG, Oyama K, Taguchi M, Endo T, Akai T, Isaji T, Hoshina K.
Title A Radiation-Crosslinked Gelatin Hydrogel That Promotes Tissue Incorporation of an Expanded Polytetrafluoroethylene Vascular Graft in Rats.
Journal Biomolecules
Abstract A prosthetic vascular graft that induces perigraft tissue incorporation may effectively prevent serious sequelae such as seroma formation and infection. Radiation-crosslinked gelatin hydrogel (RXgel) mimics the chemical and physical properties of the in vivo extracellular matrix and may facilitate wound healing by promoting tissue organization. Fibroblasts cultured on RXgel actively migrated into the gel for up to 7 days. RXgels of three different degrees of hardness (Rx[10], soft; Rx[15], middle; Rx[20], hard) were prepared, and small disc-like samples of RXgels were implanted into rats. In vitro and in vivo results indicated that Rx[10] was too soft to coat vascular grafts. Thus, expanded polytetrafluoroethylene (ePTFE) vascular grafts coated with RXgel were developed using Rx[15] and Rx[20] gels, and ring-shaped slices of the graft were implanted into rats. Alpha-smooth muscle actin (αSMA) and type III collagen (Col-III) levels were detected by immunohistochemistry. Immunohistochemical staining for αSMA and Col-III demonstrated that RXgel-coated vascular grafts induced more granulation tissue than non-coated grafts on days 14 and 28 after implantation. RXgel-coated ePTFE vascular grafts may provide a solution for patients by reducing poor perigraft tissue incorporation.
Volume 11(8)
Published 2021-7-27
DOI 10.3390/biom11081105
PII biom11081105
PMID 34439772
PMC PMC8391276
MeSH 3T3 Cells Actins / metabolism Animals Blood Vessel Prosthesis* Cell Movement Coated Materials, Biocompatible Cross-Linking Reagents / chemistry Fibroblasts / metabolism* Gelatin / chemistry* Hydrogels / chemistry* Hyperplasia Immunohistochemistry Male Mice Polytetrafluoroethylene / chemistry* Rats Rats, Sprague-Dawley Seroma / pathology Vascular Grafting / instrumentation*
IF 4.082
Human and Animal Cells 3T3 Swiss Albino(RCB1642)