Reference - Detail
| RRC ID | 69786 |
|---|---|
| Author | Sakai S, Kotani T, Harada R, Goto R, Morita T, Bouissil S, Dubessay P, Pierre G, Michaud P, El Boutachfaiti R, Nakahata M, Kojima M, Petit E, Delattre C. |
| Title | Development of phenol-grafted polyglucuronic acid and its application to extrusion-based bioprinting inks. |
| Journal | Carbohydr Polym |
| Abstract |
In this present work, we developed a phenol grafted polyglucuronic acid (PGU) and investigated the usefulness in tissue engineering field by using this derivative as a bioink component allowing gelation in extrusion-based 3D bioprinting. The PGU derivative was obtained by conjugating with tyramine, and the aqueous solution of the derivative was curable through a horseradish peroxidase (HRP)-catalyzed reaction. From 2.0 w/v% solution of the derivative containing 5 U/mL HRP, hydrogel constructs were successfully obtained with a good shape fidelity to blueprints. Mouse fibroblasts and human hepatoma cells enclosed in the printed constructs showed about 95% viability the day after printing and survived for 11 days of study without a remarkable decrease in viability. These results demonstrate the great potential of the PGU derivative in tissue engineering field especially as an ink component of extrusion-based 3D bioprinting. |
| Volume | 277 |
| Pages | 118820 |
| Published | 2022-2-1 |
| DOI | 10.1016/j.carbpol.2021.118820 |
| PII | S0144-8617(21)01207-8 |
| PMID | 34893237 |
| MeSH | Animals Bioprinting* Cell Line Glucuronic Acid / chemical synthesis Glucuronic Acid / chemistry* Glucuronic Acid / isolation & purification Ink* Mice Molecular Structure Polymers / chemical synthesis Polymers / chemistry* Polymers / isolation & purification |
| IF | 7.182 |
| Resource | |
| Human and Animal Cells | 10T1/2(RCB0247) Hep G2(RCB1886) |