| RRC ID |
69795
|
| 著者 |
Hozaka Y, Kita Y, Yasudome R, Tanaka T, Wada M, Idichi T, Tanabe K, Asai S, Moriya S, Toda H, Mori S, Kurahara H, Ohtsuka T, Seki N.
|
| タイトル |
RNA-Sequencing Based microRNA Expression Signature of Colorectal Cancer: The Impact of Oncogenic Targets Regulated by miR-490-3p.
|
| ジャーナル |
Int J Mol Sci
|
| Abstract |
To elucidate novel aspects of the molecular pathogenesis of colorectal cancer (CRC), we have created a new microRNA (miRNA) expression signature based on RNA-sequencing. Analysis of the signature showed that 84 miRNAs were upregulated, and 70 were downregulated in CRC tissues. Interestingly, our signature indicated that both guide and passenger strands of some miRNAs were significantly dysregulated in CRC tissues. These findings support our earlier data demonstrating the involvement of miRNA passenger strands in cancer pathogenesis. Our study focused on downregulated miR-490-3p and investigated its tumor-suppressive function in CRC cells. We successfully identified a total of 38 putative oncogenic targets regulated by miR-490-3p in CRC cells. Among these targets, the expression of three genes (IRAK1: p = 0.0427, FUT1: p = 0.0468, and GPRIN2: p = 0.0080) significantly predicted 5-year overall survival of CRC patients. Moreover, we analyzed the direct regulation of IRAK1 by miR-490-3p, and its resultant oncogenic function in CRC cells. Thus, we have clarified a part of the molecular pathway of CRC based on the action of tumor-suppressive miR-490-3p. This new miRNA expression signature of CRC will be a useful tool for elucidating new molecular pathogenesis in this disease.
|
| 巻・号 |
22(18)
|
| 公開日 |
2021-9-13
|
| DOI |
10.3390/ijms22189876
|
| PII |
ijms22189876
|
| PMID |
34576039
|
| PMC |
PMC8469425
|
| MeSH |
Aged
Aged, 80 and over
Biomarkers, Tumor / genetics*
Cell Line, Tumor
Cell Movement / genetics
Cell Proliferation / genetics
Colorectal Neoplasms / epidemiology
Colorectal Neoplasms / genetics*
Colorectal Neoplasms / pathology
Disease-Free Survival
Female
Gene Expression Regulation, Neoplastic / genetics
Humans
Interleukin-1 Receptor-Associated Kinases / genetics*
Male
MicroRNAs / genetics*
RNA-Seq
Transcriptome / genetics
|
| IF |
4.556
|
| リソース情報 |
| ヒト・動物細胞 |
HCT116(RCB2979) |
