論文 - 詳細
| RRC ID | 69799 |
|---|---|
| 著者 | Rawat SS, Gallo SA, Eaton J, Martin TD, Ablan S, KewalRamani VN, Wang JM, Blumenthal R, Puri A. |
| タイトル | Elevated expression of GM3 in receptor-bearing targets confers resistance to human immunodeficiency virus type 1 fusion. |
| ジャーナル | J Virol |
| Abstract |
GM3, a major ganglioside of T lymphocytes, promotes human immunodeficiency virus type 1 (HIV-1) entry via interactions with HIV-1 receptors and the viral envelope glycoprotein (Env). Increased GM3 levels in T lymphocytes and the appearance of anti-GM3 antibodies in AIDS patients have been reported earlier. In this study, we investigated the effect of GM3 regulation on HIV-1 entry by utilizing a mouse cell line (B16F10), which expresses exceptionally high levels of GM3. Strikingly, B16 cells bearing CD4, CXCR4, and/or CCR5 were highly resistant to CD4-dependent HIV-1 Env-mediated membrane fusion. In contrast, these targets supported membrane fusion mediated by CD4-requiring HIV-2, SIV, and CD4-independent HIV-1 Envs. Coreceptor function was not impaired by GM3 overexpression as indicated by Ca(2+) fluxes mediated by the CXCR4 ligand SDF-1alpha and the CCR5 ligand MIP-1beta. Reduction in GM3 levels of B16 target cells resulted in a significant recovery of CD4-dependent HIV-1 Env-mediated fusion. We propose that GM3 in the plasma membrane blocks HIV-1 Env-mediated fusion by interfering with the lateral association of HIV-1 receptors. Our findings offer a novel mechanism of interplay between membrane lipids and receptors by which host cells may escape viral infections. |
| 巻・号 | 78(14) |
| ページ | 7360-8 |
| 公開日 | 2004-7-1 |
| DOI | 10.1128/JVI.78.14.7360-7368.2004 |
| PII | 78/14/7360 |
| PMID | 15220409 |
| PMC | PMC434090 |
| MeSH | Animals CD4 Antigens / metabolism Cell Line, Tumor G(M3) Ganglioside / metabolism* G(M3) Ganglioside / pharmacology Gene Products, env / metabolism HIV-1 / pathogenicity* HIV-2 / pathogenicity HeLa Cells Humans Membrane Fusion / drug effects* Mice NIH 3T3 Cells Receptors, HIV / chemistry Receptors, HIV / metabolism* Simian Immunodeficiency Virus / pathogenicity |
| IF | 4.501 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | GM95(RCB1026) |