Reference - Detail
| RRC ID | 69830 |
|---|---|
| Author | Tavana H, Mosadegh B, Zamankhan P, Grotberg JB, Takayama S. |
| Title | Microprinted feeder cells guide embryonic stem cell fate. |
| Journal | Biotechnol Bioeng |
| Abstract |
We introduce a non-contact approach to microprint multiple types of feeder cells in a microarray format using immiscible aqueous solutions of two biopolymers. Droplets of cell suspension in the denser aqueous phase are printed on a substrate residing within a bath of the immersion aqueous phase. Due to their affinity to the denser phase, cells remain localized within the drops and adhere to regions of the substrate underneath the drops. We show the utility of this technology for creating duplex heterocellular stem cell niches by printing two different support cell types on a gel surface and overlaying them with mouse embryonic stem cells (mESCs). As desired, the type of printed support cell spatially direct the fate of overlaid mESCs. Interestingly, we found that interspaced mESCs colonies on differentiation-inducing feeder cells show enhanced neuronal differentiation and give rise to dense networks of neurons. This cell printing technology provides unprecedented capabilities to efficiently identify the role of various feeder cells in guiding the fate of stem cells. |
| Volume | 108(10) |
| Pages | 2509-16 |
| Published | 2011-10-1 |
| DOI | 10.1002/bit.23190 |
| PMID | 21538333 |
| PMC | PMC3183395 |
| MeSH | Animals Cell Differentiation* Cell Line Coculture Techniques* / instrumentation Coculture Techniques* / methods Embryonic Stem Cells* / cytology Embryonic Stem Cells* / metabolism Feeder Cells* / cytology Feeder Cells* / metabolism Mice Microarray Analysis* Neurons* / cytology Neurons* / metabolism |
| IF | 4.002 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Blogs |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | C2C12(RCB0987) |