RRC ID 69843
著者 Auer S, Hable V, Greubel C, Drexler GA, Schmid TE, Belka C, Dollinger G, Friedl AA.
タイトル Survival of tumor cells after proton irradiation with ultra-high dose rates.
ジャーナル Radiat Oncol
Abstract BACKGROUND:Laser acceleration of protons and heavy ions may in the future be used in radiation therapy. Laser-driven particle beams are pulsed and ultra high dose rates of >10⁹ Gy s⁻¹ may be achieved. Here we compare the radiobiological effects of pulsed and continuous proton beams.
METHODS:The ion microbeam SNAKE at the Munich tandem accelerator was used to directly compare a pulsed and a continuous 20 MeV proton beam, which delivered a dose of 3 Gy to a HeLa cell monolayer within < 1 ns or 100 ms, respectively. Investigated endpoints were G2 phase cell cycle arrest, apoptosis, and colony formation.
RESULTS:At 10 h after pulsed irradiation, the fraction of G2 cells was significantly lower than after irradiation with the continuous beam, while all other endpoints including colony formation were not significantly different. We determined the relative biological effectiveness (RBE) for pulsed and continuous proton beams relative to x-irradiation as 0.91 ± 0.26 and 0.86 ± 0.33 (mean and SD), respectively.
CONCLUSIONS:At the dose rates investigated here, which are expected to correspond to those in radiation therapy using laser-driven particles, the RBE of the pulsed and the (conventional) continuous irradiation mode do not differ significantly.
巻・号 6
ページ 139
公開日 2011-10-18
DOI 10.1186/1748-717X-6-139
PII 1748-717X-6-139
PMID 22008289
PMC PMC3215966
MeSH Apoptosis Cell Cycle Cell Survival Flow Cytometry HeLa Cells Heavy Ions Humans Ions Lasers Microscopy, Fluorescence / methods Neoplasms / pathology Neoplasms / radiotherapy* Particle Accelerators Protons Radiotherapy / methods Relative Biological Effectiveness Time Factors X-Rays
IF 2.817
リソース情報
ヒト・動物細胞 HeLa(RCB0007)