| RRC ID |
69844
|
| 著者 |
Fujii Y, Koizumi WC, Imai T, Yokobori M, Matsuo T, Oka K, Hotta K, Okajima T.
|
| タイトル |
Spatiotemporal dynamics of single cell stiffness in the early developing ascidian chordate embryo.
|
| ジャーナル |
Commun Biol
|
| Abstract |
During the developmental processes of embryos, cells undergo massive deformation and division that are regulated by mechanical cues. However, little is known about how embryonic cells change their mechanical properties during different cleavage stages. Here, using atomic force microscopy, we investigated the stiffness of cells in ascidian embryos from the fertilised egg to the stage before gastrulation. In both animal and vegetal hemispheres, we observed a Rho kinase (ROCK)-independent cell stiffening that the cell stiffness exhibited a remarkable increase at the timing of cell division where cortical actin filaments were organized. Furthermore, in the vegetal hemisphere, we observed another mechanical behaviour, i.e., a ROCK-associated cell stiffening, which was retained even after cell division or occurred without division and propagated sequentially toward adjacent cells, displaying a characteristic cell-to-cell mechanical variation. The results indicate that the mechanical properties of embryonic cells are regulated at the single cell level in different germ layers.
|
| 巻・号 |
4(1)
|
| ページ |
341
|
| 公開日 |
2021-3-16
|
| DOI |
10.1038/s42003-021-01869-w
|
| PII |
10.1038/s42003-021-01869-w
|
| PMID |
33727646
|
| PMC |
PMC7966737
|
| MeSH |
Actin Cytoskeleton / metabolism*
Animals
Cell Cycle Checkpoints
Ciona intestinalis / embryology*
Elastic Modulus
Embryo, Nonmammalian / cytology
Embryo, Nonmammalian / enzymology*
Embryonic Development
Mechanotransduction, Cellular*
Microscopy, Atomic Force
Mitosis
Myosins / metabolism
Single-Cell Analysis
Time Factors
rho-Associated Kinases / metabolism*
|
| IF |
4.165
|
| リソース情報 |
| カタユウレイボヤ・(ニッポンウミシダ) |
|
