RRC ID 69851
Author Fujimoto M, Kitamura H.
Title Application of the colorimetric loop-mediated isothermal amplification (LAMP) technique for genotyping Cre-driver mice.
Journal J Vet Med Sci
Abstract The Cre-loxP system is widely used to investigate the cell-type specific roles of genes of interest. Cre-driver mice are required for cell-type specific knockout during the Cre-loxP reaction. To maintain Cre-driver mouse strains, Polymerase chain reaction (PCR)-oriented genotyping targeting the Cre gene cassette is usually conducted. In this study, we instead applied a colorimetric loop-mediated isothermal amplification (LAMP) method for Cre-genotyping. Among four sets of primers designed by the in silico program, one set effectively amplified the Cre cassette of three Cre-driver strains, but not of C57BL/6 mice. This LAMP-oriented method reduces assay time by less than half compared to the PCR-based method, and can be carried out using a conventional isothermal incubator. Applying this LAMP method may accelerate genotyping of Cre-driver mice.
Volume 84(4)
Pages 507-510
Published 2022-4-13
DOI 10.1292/jvms.21-0658
PMID 35228407
PMC PMC9096032
MeSH Animals Colorimetry* / veterinary Genotype Integrases Mice Mice, Inbred C57BL Molecular Diagnostic Techniques Nucleic Acid Amplification Techniques* / methods Nucleic Acid Amplification Techniques* / veterinary Sensitivity and Specificity
IF 1.049
Mice RBRC01386