RRC ID 69877
Author Lee CG, Kim J, Yun SH, Hwang S, Jeon H, Park E, Jeong SY.
Title Anti-Osteoporotic Effect of Morroniside on Osteoblast and Osteoclast Differentiation In Vitro and Ovariectomized Mice In Vivo.
Journal Int J Mol Sci
Abstract Bone remodeling is a continuous process of bone synthesis and destruction that is regulated by osteoblasts and osteoclasts. Here, we investigated the anti-osteoporotic effects of morroniside in mouse preosteoblast MC3T3-E1 cells and mouse primary cultured osteoblasts and osteoclasts in vitro and ovariectomy (OVX)-induced mouse osteoporosis in vivo. Morroniside treatment enhanced alkaline phosphatase activity and positively stained cells via upregulation of osteoblastogenesis-associated genes in MC3T3-E1 cell lines and primary cultured osteoblasts. However, morroniside inhibited tartrate-resistant acid phosphatase activity and TRAP-stained multinucleated positive cells via downregulation of osteoclast-mediated genes in primary cultured monocytes. In the osteoporotic animal model, ovariectomized (OVX) mice were administered morroniside (2 or 10 mg/kg/day) for 12 weeks. Morroniside prevented OVX-induced bone mineral density (BMD) loss and reduced bone structural compartment loss in the micro-CT images. Taken together, morroniside promoted increased osteoblast differentiation and decreased osteoclast differentiation in cells, and consequently inhibited OVX-induced osteoporotic pathogenesis in mice. This study suggests that morroniside may be a potent therapeutic single compound for the prevention of osteoporosis.
Volume 22(19)
Published 2021-9-30
DOI 10.3390/ijms221910642
PII ijms221910642
PMID 34638983
PMC PMC8508973
MeSH Animals Bone Density / drug effects Bone Density Conservation Agents / administration & dosage* Bone Remodeling / drug effects Cell Differentiation / drug effects* Cell Line Cell Survival / drug effects Cornus / chemistry* Disease Models, Animal Female Glycosides / administration & dosage* Mice Mice, Inbred ICR Osteoblasts / drug effects Osteoblasts / metabolism* Osteoclasts / drug effects Osteoclasts / metabolism* Osteogenesis / drug effects Osteoporosis / etiology* Osteoporosis / metabolism Osteoporosis / therapy* Ovariectomy / adverse effects* Phytotherapy / methods* Plant Extracts / administration & dosage*
IF 4.556
Human and Animal Cells MC3T3-E1(RCB1126)