| RRC ID |
69877
|
| 著者 |
Lee CG, Kim J, Yun SH, Hwang S, Jeon H, Park E, Jeong SY.
|
| タイトル |
Anti-Osteoporotic Effect of Morroniside on Osteoblast and Osteoclast Differentiation In Vitro and Ovariectomized Mice In Vivo.
|
| ジャーナル |
Int J Mol Sci
|
| Abstract |
Bone remodeling is a continuous process of bone synthesis and destruction that is regulated by osteoblasts and osteoclasts. Here, we investigated the anti-osteoporotic effects of morroniside in mouse preosteoblast MC3T3-E1 cells and mouse primary cultured osteoblasts and osteoclasts in vitro and ovariectomy (OVX)-induced mouse osteoporosis in vivo. Morroniside treatment enhanced alkaline phosphatase activity and positively stained cells via upregulation of osteoblastogenesis-associated genes in MC3T3-E1 cell lines and primary cultured osteoblasts. However, morroniside inhibited tartrate-resistant acid phosphatase activity and TRAP-stained multinucleated positive cells via downregulation of osteoclast-mediated genes in primary cultured monocytes. In the osteoporotic animal model, ovariectomized (OVX) mice were administered morroniside (2 or 10 mg/kg/day) for 12 weeks. Morroniside prevented OVX-induced bone mineral density (BMD) loss and reduced bone structural compartment loss in the micro-CT images. Taken together, morroniside promoted increased osteoblast differentiation and decreased osteoclast differentiation in cells, and consequently inhibited OVX-induced osteoporotic pathogenesis in mice. This study suggests that morroniside may be a potent therapeutic single compound for the prevention of osteoporosis.
|
| 巻・号 |
22(19)
|
| 公開日 |
2021-9-30
|
| DOI |
10.3390/ijms221910642
|
| PII |
ijms221910642
|
| PMID |
34638983
|
| PMC |
PMC8508973
|
| MeSH |
Animals
Bone Density / drug effects
Bone Density Conservation Agents / administration & dosage*
Bone Remodeling / drug effects
Cell Differentiation / drug effects*
Cell Line
Cell Survival / drug effects
Cornus / chemistry*
Disease Models, Animal
Female
Glycosides / administration & dosage*
Mice
Mice, Inbred ICR
Osteoblasts / drug effects
Osteoblasts / metabolism*
Osteoclasts / drug effects
Osteoclasts / metabolism*
Osteogenesis / drug effects
Osteoporosis / etiology*
Osteoporosis / metabolism
Osteoporosis / therapy*
Ovariectomy / adverse effects*
Phytotherapy / methods*
Plant Extracts / administration & dosage*
|
| IF |
4.556
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
