| RRC ID |
69879
|
| Author |
Jarrar H, Çetin Altındal D, Gümüşderelioğlu M.
|
| Title |
Scaffold-based osteogenic dual delivery system with melatonin and BMP-2 releasing PLGA microparticles.
|
| Journal |
Int J Pharm
|
| Abstract |
The growing safety problems about the use of bone morphogenetic protein 2 (BMP-2) is one of the recent issues that was improved by using low doses of BMP-2 with the support of other osteoinductive agents and/or using appropriate carriers. The aim of the present study is to investigate the effect of scaffold-based dual release system including melatonin (MEL) and BMP-2 loaded polylactic-co-glycolic acid (PLGA) microparticles on the osteogenic activity of pre-osteoblastic MC3T3-E1 cells. MEL and BMP-2 loaded microparticles were prepared by double emulsion solvent evaporation method in the average diameters of ~2 µm and ~11 µm, respectively and loaded into chitosan/hydroxyapatite (HAp) scaffolds. In vitro MC3T3-E1 culture studies were carried out comparatively with blank scaffolds, single (BMP-2 or MEL) releasing groups and dual (BMP-2 and MEL) releasing group. Microscopic observations and hematoxylin/eosin staining showed enhanced number of cells and dense ECM in dual release group. The expressions of differentiation markers, Runt-related transcription factor 2 (RUNX2) and alkaline phosphatase (ALP) and also mineralization were higher in dual release group than that of the other groups. Our findings showed that BMP-2 at low doses (~20 ng per scaffold) was sufficient in terms of osteogenic activity with controlled release systems where it was used in combination with MEL (~10 µg per scaffold).
|
| Volume |
600
|
| Pages |
120489
|
| Published |
2021-5-1
|
| DOI |
10.1016/j.ijpharm.2021.120489
|
| PII |
S0378-5173(21)00294-5
|
| PMID |
33744449
|
| MeSH |
Bone Morphogenetic Protein 2*
Cell Differentiation
Glycols
Melatonin*
Osteogenesis
Tissue Scaffolds
|
| IF |
4.845
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126) |
