| RRC ID |
69896
|
| Author |
Ono A, Hattori S, Kariya R, Iwanaga S, Taura M, Harada H, Suzu S, Okada S.
|
| Title |
Comparative study of human hematopoietic cell engraftment into BALB/c and C57BL/6 strain of rag-2/jak3 double-deficient mice.
|
| Journal |
J Biomed Biotechnol
|
| Abstract |
Immunodeficient mice are becoming invaluable tools in human stem cell and tumor research. In this study, we generated Rag-2/Jak3 double-deficient (Rag-2⁻/⁻Jak3⁻/⁻) mice with a C57/BL6 and Balb/c genetic background and compared the human lymphohematopoietic cell engraftment rate. Human cord blood-derived CD34+ hematopoietic stem cells were successfully engrafted into Balb/c Rag-2⁻/⁻Jak3⁻/⁻ mice; however, the engraftment rate was far lower in C57/BL6 Rag-2⁻/⁻Jak3⁻/⁻ mice. Transplantation of human peripheral blood mononuclear cells resulted in the same tendency. Thus, a Balb/c background offers superior engraftment capacity than a C57/BL6 background and provides an attractive model for human hematopoietic cell engraftment.
|
| Volume |
2011
|
| Pages |
539748
|
| Published |
2011-1-1
|
| DOI |
10.1155/2011/539748
|
| PMID |
21331358
|
| PMC |
PMC3035318
|
| MeSH |
Animals
Antigens, CD34 / biosynthesis
Antigens, CD34 / immunology
DNA-Binding Proteins / deficiency*
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / immunology
Flow Cytometry
Hematopoietic Stem Cell Transplantation*
Hematopoietic Stem Cells / immunology
Humans
Janus Kinase 3 / deficiency*
Janus Kinase 3 / genetics*
Janus Kinase 3 / immunology
K562 Cells
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
|
| IF |
1.785
|
| Resource |
| Human and Animal Cells |
K562 |
