| RRC ID |
6994
|
| Author |
Nunoura T, Hirai M, Imachi H, Miyazaki M, Makita H, Hirayama H, Furushima Y, Yamamoto H, Takai K.
|
| Title |
Kosmotoga arenicorallina sp. nov. a thermophilic and obligately anaerobic heterotroph isolated from a shallow hydrothermal system occurring within a coral reef, southern part of the Yaeyama Archipelago, Japan, reclassification of Thermococcoides shengliensis as Kosmotoga shengliensis comb. nov., and emended description of the genus Kosmotoga.
|
| Journal |
Arch Microbiol
|
| Abstract |
A novel thermophilic and sulfur-reducing bacterium, strain S304(T), was isolated from the Taketomi submarine hot spring shallow hydrothermal field located at southern part of the Yaeyama Archipelago, Japan. The cells were non-motile short thick rods or oval cocci 1.1-2.7 μm in length and 1.1-1.9 μm in width. Strain S304(T) was an obligately anaerobic heterotroph and sulfur reduction stimulates growth. Growth was observed between 50-65°C (optimum 60°C), pH 6.2-8.0 (optimum pH 7.1), 1.0-6.0% NaCl concentration (optimum 3.0%). The fatty acid composition was C(16:0) (71.4%), C(18:0) (20.9%) and C(18:1) (7.7%). The G + C content of genomic DNA was 40.8 mol%. The 16S rRNA gene sequence analysis indicated that strain S304(T) belonged to the genus Kosmotoga. Based on physiological and phylogenetic features of a new isolate, we propose new species in the genus Kosmotoga: the type strain of Kosmotoga arenicorallina sp. nov is S304(T) (=JCM 15790(T) = DSM22549(T)). Thermococcoides shengliensis 2SM-2(T) is phylogenetically associated with Kosmotoga olearia 14.5.1(T). Based on the phylogenetic relationship between Thermococcoides shengliensis 2SM-2(T) and Kosmotoga olearia 14.5.1(T), we propose the reclassification of Thermococcoides shengliensis as Kosmotoga shengliensis comb. nov. (type strain 2SM-2(T)). In addition, an emended description of the genus Kosmotoga is proposed.
|
| Volume |
192(10)
|
| Pages |
811-9
|
| Published |
2010-10-1
|
| DOI |
10.1007/s00203-010-0611-7
|
| PMID |
20694719
|
| MeSH |
Bacteria, Anaerobic / classification
Bacteria, Anaerobic / growth & development
Bacteria, Anaerobic / isolation & purification*
Bacterial Typing Techniques
Base Composition
Coral Reefs*
Culture Media
DNA, Bacterial / genetics
Genes, Bacterial
Hot Temperature
Japan
Phylogeny
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Seawater / microbiology
Sequence Analysis, DNA
Species Specificity
Sulfur-Reducing Bacteria / classification
Sulfur-Reducing Bacteria / growth & development
Sulfur-Reducing Bacteria / isolation & purification*
Water Microbiology*
|
| IF |
1.884
|
| Times Cited |
25
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 15790 |
