論文 - 詳細
| RRC ID | 69959 |
|---|---|
| 著者 | Cho HJ, Moy Y, Rudnick NA, Klein TM, Yin J, Bolar J, Hendrick C, Beatty M, Castañeda L, Kinney AJ, Jones TJ, Chilcoat ND. |
| タイトル | Development of an efficient marker-free soybean transformation method using the novel bacterium Ochrobactrum haywardense H1. |
| ジャーナル | Plant Biotechnol J |
| Abstract |
We have discovered a novel bacterium, Ochrobactrum haywardense H1 (Oh H1), which is capable of efficient plant transformation. Ochrobactrum is a new host for Agrobacterium-derived vir and T-DNA-mediated transformation. Oh H1 is a unique, non-phytopathogenic species, categorized as a BSL-1 organism. We engineered Oh H1 with repurposed Agrobacterium virulence machinery and demonstrated Oh H1 can transform numerous dicot species and at least one monocot, sorghum. We generated a cysteine auxotrophic Oh H1-8 strain containing a binary vector system. Oh H1-8 produced transgenic soybean plants with an efficiency 1.6 times that of Agrobacterium strain AGL1 and 2.9 times that of LBA4404Thy-. Oh H1-8 successfully transformed several elite Corteva soybean varieties with T0 transformation frequency up to 35%. In addition to higher transformation efficiencies, Oh H1-8 generated high-quality, transgenic events with single-copy, plasmid backbone-free insertion at frequencies higher than AGL1. The SpcN selectable marker gene is excised using a heat shock-inducible excision system resulting in marker-free transgenic events. Approximately, 24.5% of the regenerated plants contained only a single copy of the transgene and contained no vector backbone. There were no statistically significant differences in yield comparing T3 null-segregant lines to wild-type controls. We have demonstrated that Oh H1-8, combined with spectinomycin selection, is an efficient, rapid, marker-free and yield-neutral transformation system for elite soybean. |
| 巻・号 | 20(5) |
| ページ | 977-990 |
| 公開日 | 2022-5-1 |
| DOI | 10.1111/pbi.13777 |
| PMID | 35015927 |
| PMC | PMC9055811 |
| MeSH | Agrobacterium tumefaciens / genetics Genetic Vectors Ochrobactrum* / genetics Plants, Genetically Modified Soybeans* / genetics Transformation, Genetic |
| IF | 8.154 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 65 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00001 |