| RRC ID |
69984
|
| 著者 |
Kanemaru H, Mizukami Y, Kaneko A, Kajihara I, Fukushima S.
|
| タイトル |
A protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer.
|
| ジャーナル |
STAR Protoc
|
| Abstract |
Current standard assays to analyze lymphocyte-mediated antitumor cytotoxicity employ radioisotopic or fluorescent labels. However, such assays are not suitable for real-time analysis. Here we describe a protocol that facilitates the analysis of lymphocyte-mediated toxicity using a label-free, impedance-based real-time cell analyzer. This analyzer measures cellular electrical impedance, expressed as the cell index value, noninvasively and continuously. In contrast with label-dependent assays, this protocol simultaneously generates real-time killing curves useful for quantifying lymphocyte-mediated cytotoxicity in real time. For complete details on the use and execution of this protocol, please refer to Kanemaru et al. (2021).
|
| 巻・号 |
3(1)
|
| ページ |
101128
|
| 公開日 |
2022-3-18
|
| DOI |
10.1016/j.xpro.2022.101128
|
| PII |
S2666-1667(22)00008-9
|
| PMID |
35118432
|
| PMC |
PMC8792266
|
| MeSH |
Biological Assay*
Cytotoxicity, Immunologic*
Electric Impedance
|
| リソース情報 |
| ヒト・動物細胞 |
B16F1(RCB2649) |
