論文 - 詳細
| RRC ID | 69986 |
|---|---|
| 著者 | Arae T, Morita K, Imahori R, Suzuki Y, Yasuda S, Sato T, Yamaguchi J, Chiba Y. |
| タイトル | Identification of Arabidopsis CCR4-NOT Complexes with Pumilio RNA-Binding Proteins, APUM5 and APUM2. |
| ジャーナル | Plant Cell Physiol |
| Abstract |
CCR4/CAF1 are widely conserved deadenylases in eukaryotes. They form a large complex that includes NOT1 as a scaffold protein and various NOT proteins that are core components of multiple levels of gene expression control. The CCR4-NOT complex also contains several RNA-binding proteins as accessory proteins, which are required for target recognition by CCR4/CAF1 deadenylases. AtCCR4a/b, orthologs of human CCR4 in Arabidopsis, have various physiological effects. AtCCR4 isoforms are likely to have specific target mRNAs related to each physiological effect; however, AtCCR4 does not have RNA-binding capability. Therefore, identifying factors that interact with AtCCR4a/b is indispensable to understand its function as a regulator of gene expression, as well as the target mRNA recognition mechanism. Here, we identified putative components of the AtCCR4-NOT complex using co-immunoprecipitation in combination with mass spectrometry using FLAG-tagged AtCCR4b and subsequent verification with a yeast two-hybrid assay. Interestingly, four of 11 AtCAF1 isoforms interacted with both AtCCR4b and AtNOT1, whereas two isoforms interacted only with AtNOT1 in yeast two-hybrid assays. These results imply that Arabidopsis has multiple CCR4-NOT complexes with various combinations of deadenylases. We also revealed that the RNA-binding protein Arabidopsis Pumilio 5 and 2 interacted with AtCCR4a/b in the cytoplasm with a few foci. |
| 巻・号 | 60(9) |
| ページ | 2015-2025 |
| 公開日 | 2019-9-1 |
| DOI | 10.1093/pcp/pcz089 |
| PII | 5489917 |
| PMID | 31093672 |
| MeSH | Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cytoplasm / metabolism Phylogeny Protein Isoforms RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Receptors, CCR4 / genetics Repressor Proteins / genetics Repressor Proteins / metabolism* Two-Hybrid System Techniques |
| IF | 4.062 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00008 |