論文 - 詳細
| RRC ID | 69990 |
|---|---|
| 著者 | Plessy C, Bertin N, Takahashi H, Simone R, Salimullah M, Lassmann T, Vitezic M, Severin J, Olivarius S, Lazarevic D, Hornig N, Orlando V, Bell I, Gao H, Dumais J, Kapranov P, Wang H, Davis CA, Gingeras TR, Kawai J, Daub CO, Hayashizaki Y, Gustincich S, Carninci P. |
| タイトル | Linking promoters to functional transcripts in small samples with nanoCAGE and CAGEscan. |
| ジャーナル | Nat Methods |
| Abstract |
Large-scale sequencing projects have revealed an unexpected complexity in the origins, structures and functions of mammalian transcripts. Many loci are known to produce overlapping coding and noncoding RNAs with capped 5' ends that vary in size. Methods to identify the 5' ends of transcripts will facilitate the discovery of new promoters and 5' ends derived from secondary capping events. Such methods often require high input amounts of RNA not obtainable from highly refined samples such as tissue microdissections and subcellular fractions. Therefore, we developed nano-cap analysis of gene expression (nanoCAGE), a method that captures the 5' ends of transcripts from as little as 10 ng of total RNA, and CAGEscan, a mate-pair adaptation of nanoCAGE that captures the transcript 5' ends linked to a downstream region. Both of these methods allow further annotation-agnostic studies of the complex human transcriptome. |
| 巻・号 | 7(7) |
| ページ | 528-34 |
| 公開日 | 2010-7-1 |
| DOI | 10.1038/nmeth.1470 |
| PII | nmeth.1470 |
| PMID | 20543846 |
| PMC | PMC2906222 |
| MeSH | Gene Expression Profiling* Gene Expression Regulation / physiology* Genome, Human Humans Nanotechnology / methods* Promoter Regions, Genetic / physiology* RNA / genetics RNA / metabolism* |
| IF | 30.822 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 20 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB1648) |