RRC ID 70025
著者 Do KM, Shin MK, Kodama T, Win NN, Prema P, Nguyen HM, Hayakawa Y, Morita H.
タイトル Flavanols and Flavanes from Crinum asiaticum and Their Effects on LPS Signaling Pathway Through the Inhibition of NF-κB Activation.
ジャーナル Planta Med
Abstract Three new flavanols, (2R,3S)-7-methoxy-flavan-3-ol (1: ), (2R,3S)-7-hydroxy-flavan-3-ol (2: ), and (2R,3S)-2'-hydroxy-7-methoxy-flavan-3-ol (3: ), together with two known flavans (4: and 5: ), were isolated from the chloroform extract of Crinum asiaticum. Their structures were elucidated by various spectroscopic methods, including 1D and 2D NMR, HR-ESI-MS, and CD data. The isolated compounds 1: and 3: -5: showed inhibitory activity toward LPS-induced nitric oxide (NO) production. Further investigation of the NF-κB pathway mechanisms indicated that 1: and 3: -5: inhibited the LPS-induced IL-6 production and p65 subunit phosphorylation of NF-κB in RAW264.7 cells, with an effective dose of 10 µM.
巻・号 88(11)
ページ 913-920
公開日 2022-9-1
DOI 10.1055/a-1585-5877
PMID 34474490
MeSH Animals Crinum* / metabolism Flavonoids / chemistry* Lipopolysaccharides / pharmacology Mice NF-kappa B* / metabolism Nitric Oxide / metabolism Nitric Oxide Synthase Type II / metabolism Polyphenols RAW 264.7 Cells Signal Transduction
IF 2.687
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)