| RRC ID |
7004
|
| Author |
Tsuruta T, Inoue R, Iwanaga T, Hara H, Yajima T.
|
| Title |
Development of a method for the identification of S-IgA-coated bacterial composition in mouse and human feces.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
Some commensal intestinal bacteria in humans and mice are coated with secretory immunoglobulin A (S-IgA). It has been suggested that the S-IgA coating of commensal bacteria does not occur at random and thus identification of S-IgA-coated bacterial genera/species should help in elucidating the interaction between S-IgA and commensal intestinal bacteria, but no method of identifying the genera/species of S-IgA-coated bacteria has been established. To identify S-IgA-coated bacterial composition, we developed a method combining immunohistochemical detection of S-IgA and subsequent 16S rRNA targeted fluorescence in situ hybridization (FISH) analysis. Human and mouse fecal S-IgA coated bacterial composition was evaluated by this newly developed method with 10 frequently-used FISH probes. Fecal S-IgA-coated bacterial composition was successfully analyzed by this method, and this suggests that Enterobacteriaceae is preferably coated with S-IgA, whereas Bacteroides/Prevotella and Lactobacillus/Enterococcus groups appear to be poorly coated with S-IgA.
|
| Volume |
74(5)
|
| Pages |
968-73
|
| Published |
2010-1-1
|
| DOI |
10.1271/bbb.90801
|
| PII |
JST.JSTAGE/bbb/90801
|
| PMID |
20460734
|
| MeSH |
Adult
Animals
Bacteria / classification*
Bacteria / genetics
Bacteria / isolation & purification
Bacteria / metabolism*
Bacterial Typing Techniques / methods*
Base Sequence
Feces / microbiology*
Female
Glass
Humans
Immunoglobulin A, Secretory / metabolism*
In Situ Hybridization, Fluorescence
Mice
Reproducibility of Results
|
| IF |
1.516
|
| Times Cited |
15
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| General Microbes |
JCM 5827
JCM 1395
JCM 11662
JCM 8129
JCM 14654
JCM 9981
JCM 1772 |
