論文 - 詳細
| RRC ID | 70113 |
|---|---|
| 著者 | Kieu TQ, Tazawa K, Kawashima N, Noda S, Fujii M, Nara K, Hashimoto K, Han P, Okiji T. |
| タイトル | Kinetics of LYVE-1-positive M2-like macrophages in developing and repairing dental pulp in vivo and their pro-angiogenic activity in vitro. |
| ジャーナル | Sci Rep |
| Abstract |
Tissue-resident macrophages expressing lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) are found in multiple tissues and organs. We aimed to evaluate the dynamics and biological functions of LYVE-1+ macrophages in dental pulp during post-injury tissue remodeling. Immunofluorescence staining of mouse embryos revealed that LYVE-1+ macrophages colonized dental pulp before birth. In mature rat molar dental pulp, LYVE-1+ macrophages were the main subset of macrophages expressing CD163, an M2 marker, and were distributed throughout the tissue. In response to dental pulp injury induced by cavity preparation, LYVE-1+ macrophages quickly disappeared from the affected area of the pulp and gradually repopulated during the wound healing process. RAW264.7 mouse macrophages cultured with a mixture of macrophage colony-stimulating factor, interleukin-4, and dexamethasone increased LYVE-1 expression, whereas lipopolysaccharide-stimulation decreased LYVE-1 expression. Enforced expression of Lyve1 in RAW264.7 cells resulted in increased mRNA expression of matrix metalloproteinase 2 (Mmp2), Mmp9, and vascular endothelial growth factor A (Vegfa). Lyve1-expressing macrophages promoted the migration and tube formation of human umbilical vein endothelial cells. In conclusion, LYVE-1+ tissue-resident M2-like macrophages in dental pulp showed dynamism in response to pulp injury, and possibly play an important role in angiogenesis during wound healing and tissue remodeling. |
| 巻・号 | 12(1) |
| ページ | 5176 |
| 公開日 | 2022-3-25 |
| DOI | 10.1038/s41598-022-08987-3 |
| PII | 10.1038/s41598-022-08987-3 |
| PMID | 35338195 |
| PMC | PMC8956626 |
| MeSH | Animals Dental Pulp / metabolism Endothelial Cells / metabolism Kinetics Macrophages / metabolism Matrix Metalloproteinase 2* / metabolism Membrane Transport Proteins / metabolism Mice Rats Vascular Endothelial Growth Factor A* / metabolism |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |