| RRC ID |
70113
|
| 著者 |
Kieu TQ, Tazawa K, Kawashima N, Noda S, Fujii M, Nara K, Hashimoto K, Han P, Okiji T.
|
| タイトル |
Kinetics of LYVE-1-positive M2-like macrophages in developing and repairing dental pulp in vivo and their pro-angiogenic activity in vitro.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Tissue-resident macrophages expressing lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) are found in multiple tissues and organs. We aimed to evaluate the dynamics and biological functions of LYVE-1+ macrophages in dental pulp during post-injury tissue remodeling. Immunofluorescence staining of mouse embryos revealed that LYVE-1+ macrophages colonized dental pulp before birth. In mature rat molar dental pulp, LYVE-1+ macrophages were the main subset of macrophages expressing CD163, an M2 marker, and were distributed throughout the tissue. In response to dental pulp injury induced by cavity preparation, LYVE-1+ macrophages quickly disappeared from the affected area of the pulp and gradually repopulated during the wound healing process. RAW264.7 mouse macrophages cultured with a mixture of macrophage colony-stimulating factor, interleukin-4, and dexamethasone increased LYVE-1 expression, whereas lipopolysaccharide-stimulation decreased LYVE-1 expression. Enforced expression of Lyve1 in RAW264.7 cells resulted in increased mRNA expression of matrix metalloproteinase 2 (Mmp2), Mmp9, and vascular endothelial growth factor A (Vegfa). Lyve1-expressing macrophages promoted the migration and tube formation of human umbilical vein endothelial cells. In conclusion, LYVE-1+ tissue-resident M2-like macrophages in dental pulp showed dynamism in response to pulp injury, and possibly play an important role in angiogenesis during wound healing and tissue remodeling.
|
| 巻・号 |
12(1)
|
| ページ |
5176
|
| 公開日 |
2022-3-25
|
| DOI |
10.1038/s41598-022-08987-3
|
| PII |
10.1038/s41598-022-08987-3
|
| PMID |
35338195
|
| PMC |
PMC8956626
|
| MeSH |
Animals
Dental Pulp / metabolism
Endothelial Cells / metabolism
Kinetics
Macrophages / metabolism
Matrix Metalloproteinase 2* / metabolism
Membrane Transport Proteins / metabolism
Mice
Rats
Vascular Endothelial Growth Factor A* / metabolism
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
