| RRC ID |
70120
|
| Author |
Kusama K, Suzuki Y, Kurita E, Kawarasaki T, Obara K, Okumura F, Kamura T, Nakatsukasa K.
|
| Title |
Dot6/Tod6 degradation fine-tunes the repression of ribosome biogenesis under nutrient-limited conditions.
|
| Journal |
iScience
|
| Abstract |
Ribosome biogenesis (Ribi) is a complex and energy-consuming process, and should therefore be repressed under nutrient-limited conditions to minimize unnecessary cellular energy consumption. In yeast, the transcriptional repressors Dot6 and Tod6 are phosphorylated and inactivated by the TORC1 pathway under nutrient-rich conditions, but are activated and repress ∼200 Ribi genes under nutrient-limited conditions. However, we show that in the presence of rapamycin or under nitrogen starvation conditions, Dot6 and Tod6 were readily degraded by the proteasome in a SCFGrr1 and Tom1 ubiquitin ligase-dependent manner, respectively. Moreover, promiscuous accumulation of Dot6 and Tod6 excessively repressed Ribi gene expression as well as translation activity and caused a growth defect in the presence of rapamycin. Thus, we propose that degradation of Dot6 and Tod6 is a novel mechanism to ensure an appropriate level of Ribi gene expression and thereby fine-tune the repression of Ribi and translation activity for cell survival under nutrient-limited conditions.
|
| Volume |
25(3)
|
| Pages |
103986
|
| Published |
2022-3-18
|
| DOI |
10.1016/j.isci.2022.103986
|
| PII |
S2589-0042(22)00256-5
|
| PMID |
35310337
|
| PMC |
PMC8924686
|
| IF |
4.447
|
| Resource |
| Yeast |
BYP1804, BYP1805 |
