論文 - 詳細
| RRC ID | 70179 |
|---|---|
| 著者 | Shimizu K, Suzuki H, Uemura T, Nozawa A, Desaki Y, Hoshino R, Yoshida A, Abe H, Nishiyama M, Nishiyama C, Sawasaki T, Arimura GI. |
| タイトル | Immune gene activation by NPR and TGA transcriptional regulators in the model monocot Brachypodium distachyon. |
| ジャーナル | Plant J |
| Abstract |
The nonexpressor of pathogenesis-related (NPR) gene family is well known to play a crucial role in transactivation of TGA transcription factors for salicylic acid (SA)-responsive genes, including pathogenesis-related protein 1 (PR1), during plants' immune response after pathogen attack in the model dicot Arabidopsis thaliana. However, little is known about NPR gene functions in monocots. We therefore explored the functions of NPRs in SA signaling in the model monocot Brachypodium distachyon. BdNPR1 and BdNPR2/3 share structural similarities with A. thaliana AtNPR1/2 and AtNPR3/4 subfamilies, respectively. The transcript level of BdNPR2 but not BdNPR1/3 appeared to be positively regulated in leaves in response to methyl salicylate. Reporter assays in protoplasts showed that BdNPR2 positively regulated BdTGA1-mediated activation of PR1. This transactivation occurred in an SA-dependent manner through SA binding at Arg468 of BdNPR2. In contrast, BdNPR1 functioned as a suppressor of BdNPR2/BdTGA1-mediated transcription of PR1. Collectively, our findings reveal that the TGA-promoted transcription of SA-inducible PR1 is orchestrated by the activator BdNPR2 and the repressor BdNPR1, which function competitively in B. distachyon. |
| 巻・号 | 110(2) |
| ページ | 470-481 |
| 公開日 | 2022-4-1 |
| DOI | 10.1111/tpj.15681 |
| PMID | 35061931 |
| MeSH | Arabidopsis* / genetics Arabidopsis* / metabolism Brachypodium* / genetics Brachypodium* / metabolism Gene Expression Regulation, Plant / genetics Plant Proteins / genetics Plant Proteins / metabolism Salicylic Acid / metabolism Transcription Factors / genetics Transcription Factors / metabolism Transcriptional Activation / genetics |
| IF | 6.141 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pdb17757 pdb07629 pdb33950 pdb15566 pdb26078 |