論文 - 詳細
| RRC ID | 70187 |
|---|---|
| 著者 | Yamamoto T, Alimu Y, Takahashi H, Kusuya Y, Hosoya K, Shigemune N, Nagai S, Yaguchi T. |
| タイトル | Isolation and Characterization of the Polyhexamethylene Biguanide Hydrochloride-Resistant Fungus, Purpureocillium lilacinum. |
| ジャーナル | Biocontrol Sci |
| Abstract |
We isolated a fungus from a 20% (= 200,000 µg/mL) aqueous solution of polyhexamethylene biguanide hydrochloride (PHMB), a widely used antimicrobial and examined its morphology and drug resistance profile. Based on the sequence of the internal transcribed spacer region of ribosomal DNA, the fungus was identified as Purpureocillium lilacinum. Although the P. lilacinum type and resistant strains showed similar morphology, the latter had extremely low PHMB susceptibility and was able to grow in 20% aqueous solution of PHMB, which eliminated the type strain. The minimum inhibitory concentration (MIC) of PHMB for the resistant strain was significantly higher than that of the type strain and other pathogenic filamentous fungi and yeasts. The susceptibility to antimicrobial agents and antifungal agents other than PHMB was similar to that of the type strain, therefore the drug resistance of the isolate was specific to PHMB. Furthermore, we sequenced the genome of the isolate to predict PHMB resistance-related genes. Despite its high resistance to PHMB, no well-known genes homologous to fungal PHMB-resistant genes were detected in the genome of the resistant strain. In summary, P. lilacinum was found to be significantly more resistant to PHMB than previously reported, via an unidentified mechanism of drug resistance. |
| 巻・号 | 26(3) |
| ページ | 157-166 |
| 公開日 | 2021-1-1 |
| DOI | 10.4265/bio.26.157 |
| PMID | 34556618 |
| MeSH | Biguanides* / pharmacology Fungi* Hypocreales Microbial Sensitivity Tests |
| IF | 0.833 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原真核微生物 | Purpureocillium lilacinum IFM 63780 |