RRC ID 70210
Author Okamura Y, Sato A, Kawaguchi L, Nagano AJ, Murakami M, Vogel H, Kroymann J.
Title Microevolution of Pieris butterfly genes involved in host plant adaptation along a host plant community cline.
Journal Mol Ecol
Abstract Herbivorous insects have evolved counteradaptations to overcome the chemical defenses of their host plants. Several of these counteradaptations have been elucidated at the molecular level, in particular for insects specialized on cruciferous host plants. While the importance of these counteradaptations for host plant colonization is well established, little is known about their microevolutionary dynamics in the field. In particular, it is not known whether and how host plant diversity shapes diversity in insect counteradaptations. In this study, we examine patterns of host plant use and insect counteradaptation in three Pieris butterfly species across Japan. The larvae of these butterflies express nitrile-specifier protein (NSP) and its paralog major allergen (MA) in their gut to overcome the highly diversified glucosinolate-myrosinase defense system of their cruciferous host plants. Pieris napi and Pieris melete colonize wild Brassicaceae whereas Pieris rapae typically uses cultivated Brassica as a host, regardless of the local composition of wild crucifers. As expected, NSP and MA diversity was independent of the local composition of wild Brassicaceae in P. rapae. In contrast, NSP diversity correlated with local host plant diversity in both species that preferred wild Brassicaceae. P. melete and P. napi both revealed two distinct major NSP alleles, which shaped diversity among local populations, albeit with different evolutionary trajectories. In comparison, MA showed no indication for local adaptation. Altogether, MA appeared to be evolutionary more conserved than NSP, suggesting that both genes play different roles in diverting host plant chemical defense.
Volume 31(11)
Pages 3083-3097
Published 2022-6-1
DOI 10.1111/mec.16447
PMID 35364616
MeSH Animals Brassicaceae* / chemistry Butterflies* / genetics Ericaceae* Glucosinolates / genetics Insecta Larva / genetics
IF 5.163
Resource
GBIF Plant Specimen Database of Tama Forest Science Garden, Forestry and Forest Products Research Institute, Japan Vascular plant specimens of Akita Prefectural Museum Herbarium, University Archives and Collections, Fukushima University, Japan (FKSE) Angiospermatatophytina Collection of Saitama Museum of Natural History Plant specimens of Oiso Municipal Museum Vascular Plant Collection of Natural History Museum and Institute, Chiba Plant Specimens of Kurashiki Museum of Natural History Herbarium Specimens of Tokushima Prefectural Museum, Japan Vascular Plants Collection of Sagamihara City Museum Plant specimens in the Museum of Nature and Human Activities, Hyogo Prefecture, Japan Plant specimens deposited in Osaka Museum of Natural History, Japan Vascular Plant Specimens of Kochi Prefectural Makino Botanical Garden Vascular Plant Specimen database of Kanagawa Prefectural Museum of Natural History Gunma Museum of Natural History, Vascular Plant Specimen Plant Observation Records of Japan