Reference - Detail
| RRC ID | 7027 |
|---|---|
| Author | Paściak M, Kaczyński Z, Lindner B, Holst O, Gamian A. |
| Title | Immunochemical studies of trehalose-containing major glycolipid from Tsukamurella pulmonis. |
| Journal | Carbohydr Res |
| Abstract |
The chemical structure of the major glycolipid present in the chloroform-methanol extract of bacterial biomass of Tsukamurella pulmonis is reported. This compound was purified by TLC and HPLC. The sugar analysis revealed only glucose. Detailed chemical analyses, NMR, and MALDI FT-ICR-mass spectrometric studies identified 2,3-di-O-acyl-alpha-d-glucopyranosyl-(1-->1)-alpha-d-glucopyranose as the final structure. Short branched fatty acids (4:0 or 5:0) were linked to C-3 and saturated, mono, and diunsaturated 18:0, 18:1, 18:2, 20:1, 20:2, and 20:0 to C-2 of the same glucose residue. ELISA tests revealed the weak cross-reactivity of the glycolipid with rabbit antisera against cells of T. paurometabola, Rhodococcus wratislaviensis, and Nocardiopsis dassonvillei. |
| Volume | 345(11) |
| Pages | 1570-4 |
| Published | 2010-7-19 |
| DOI | 10.1016/j.carres.2010.04.026 |
| PII | S0008-6215(10)00184-9 |
| PMID | 20510396 |
| MeSH | Actinomycetales / chemistry* Animals Carbohydrate Sequence Chloroform / chemistry Enzyme-Linked Immunosorbent Assay Glycolipids / chemistry* Glycolipids / immunology* Glycolipids / isolation & purification Immunochemistry Magnetic Resonance Spectroscopy Methanol / chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Trehalose / chemistry* |
| IF | 1.841 |
| Times Cited | 8 |
| WOS Category | CHEMISTRY, APPLIED CHEMISTRY, ORGANIC BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| General Microbes | JCM 10111 JCM 10110 JCM 10112 |