| RRC ID |
70316
|
| Author |
Male I, Ozacar AT, Fagan RR, Loring MD, Shen MC, Pace VA, Devine CA, Lawson GE, Lutservitz A, Karlstrom RO.
|
| Title |
Hedgehog Signaling Regulates Neurogenesis in the Larval and Adult Zebrafish Hypothalamus.
|
| Journal |
eNeuro
|
| Abstract |
Neurogenesis is now known to play a role in adult hypothalamic function, yet the cell-cell mechanisms regulating this neurogenesis remain poorly understood. Here, we show that Hedgehog (Hh)/Gli signaling positively regulates hypothalamic neurogenesis in both larval and adult zebrafish and is necessary and sufficient for normal hypothalamic proliferation rates. Hh-responsive radial glia represent a relatively highly proliferative precursor population that gives rise to dopaminergic, serotonergic, and GABAergic neurons. In situ and transgenic reporter analyses revealed substantial heterogeneity in cell-cell signaling within the hypothalamic niche, with slow cycling Nestin-expressing cells residing among distinct and overlapping populations of Sonic Hh (Shh)-expressing, Hh-responsive, Notch-responsive, and Wnt-responsive radial glia. This work shows for the first time that Hh/Gli signaling is a key component of the complex cell-cell signaling environment that regulates hypothalamic neurogenesis throughout life.
|
| Volume |
7(6)
|
| Published |
2020-1-1
|
| DOI |
10.1523/ENEURO.0226-20.2020
|
| PII |
ENEURO.0226-20.2020
|
| PMID |
33106384
|
| PMC |
PMC7769882
|
| MeSH |
Animals
Hedgehog Proteins* / genetics
Hedgehog Proteins* / metabolism
Hypothalamus / metabolism
Larva / metabolism
Neurogenesis
Signal Transduction
Zebrafish* / metabolism
|
| IF |
3.544
|
| Resource |
| Zebrafish |
Tg(gad1b:GFP) |
