RRC ID 70317
Author Rima M, Lattouf Y, Abi Younes M, Bullier E, Legendre P, Mangin JM, Hong E.
Title Dynamic regulation of the cholinergic system in the spinal central nervous system.
Journal Sci Rep
Abstract While the role of cholinergic neurotransmission from motoneurons is well established during neuromuscular development, whether it regulates central nervous system development in the spinal cord is unclear. Zebrafish presents a powerful model to investigate how the cholinergic system is set up and evolves during neural circuit formation. In this study, we carried out a detailed spatiotemporal analysis of the cholinergic system in embryonic and larval zebrafish. In 1-day-old embryos, we show that spinal motoneurons express presynaptic cholinergic genes including choline acetyltransferase (chata), vesicular acetylcholine transporters (vachta, vachtb), high-affinity choline transporter (hacta) and acetylcholinesterase (ache), while nicotinic acetylcholine receptor (nAChR) subunits are mainly expressed in interneurons. However, in 3-day-old embryos, we found an unexpected decrease in presynaptic cholinergic transcript expression in a rostral to caudal gradient in the spinal cord, which continued during development. On the contrary, nAChR subunits remained highly expressed throughout the spinal cord. We found that protein and enzymatic activities of presynaptic cholinergic genes were also reduced in the rostral spinal cord. Our work demonstrating that cholinergic genes are initially expressed in the embryonic spinal cord, which is dynamically downregulated during development suggests that cholinergic signaling may play a pivotal role during the formation of intra-spinal locomotor circuit.
Volume 10(1)
Pages 15338
Published 2020-9-18
DOI 10.1038/s41598-020-72524-3
PII 10.1038/s41598-020-72524-3
PMID 32948826
PMC PMC7501295
MeSH Animals Animals, Genetically Modified Central Nervous System / embryology* Central Nervous System / metabolism Choline O-Acetyltransferase / genetics Choline O-Acetyltransferase / metabolism Embryo, Nonmammalian Gene Expression Regulation, Developmental* Larva / metabolism Motor Neurons / metabolism Neurons / physiology Neurotransmitter Agents / metabolism Spinal Cord / embryology* Spinal Cord / metabolism Vesicular Acetylcholine Transport Proteins / genetics Vesicular Acetylcholine Transport Proteins / metabolism Zebrafish / embryology* Zebrafish / genetics Zebrafish Proteins / genetics Zebrafish Proteins / metabolism*
IF 3.998
Zebrafish Tg(vglut2a:loxP-DsRed-loxP-GFP)