論文 - 詳細
| RRC ID | 70403 |
|---|---|
| 著者 | Ashraf MA, Akihiro T, Ito K, Kumagai S, Sugita R, Tanoi K, Rahman A. |
| タイトル | ATP binding cassette proteins ABCG37 and ABCG33 function as potassium-independent cesium uptake carriers in Arabidopsis roots. |
| ジャーナル | Mol Plant |
| Abstract |
Radiocesium accumulated in the soil by nuclear accidents is a major environmental concern. The transport process of cesium (Cs+) is tightly linked to the indispensable plant nutrient potassium (K+) as they both belong to the group I alkali metals with similar chemical properties. Most of the transporters that had been characterized to date as Cs+ transporters are directly or indirectly linked to K+. Using a combinatorial approach of physiology, genetics, cell biology, and root uptake assay, here we identified two ATP-binding cassette (ABC) proteins, ABCG37 and ABCG33, as facilitators of Cs+ influx. A gain-of-function mutant of ABCG37 (abcg37-1) showed increased sensitivity to Cs+-induced root growth inhibition, while the double knockout mutant of ABCG33 and ABCG37 (abcg33-1abcg37-2) showed resistance, whereas the single loss-of-function mutants of ABCG33 and ABCG37 did not show any alteration in Cs+ response. In planta short-term radioactive Cs+-uptake assay along with growth and uptake assays in a heterologous system confirmed ABCG33 and ABCG37 as Cs+-uptake carriers. Potassium response and content were unaffected in the double-mutant background and yeast cells lacking potassium-uptake carriers transformed with ABCG33 and ABCG37 failed to grow in the absence of K+, confirming that Cs+ uptake by ABCG33 and ABCG37 is independent of K+. Collectively, this work identified two ABC proteins as new Cs+-influx carriers that act redundantly and independent of the K+-uptake pathway. |
| 巻・号 | 14(4) |
| ページ | 664-678 |
| 公開日 | 2021-4-5 |
| DOI | 10.1016/j.molp.2021.02.002 |
| PII | S1674-2052(21)00048-4 |
| PMID | 33588076 |
| MeSH | ATP Binding Cassette Transporter, Subfamily G / genetics ATP Binding Cassette Transporter, Subfamily G / metabolism* Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Biological Transport Cesium / metabolism* Plant Roots / genetics Plant Roots / metabolism* Potassium / metabolism* |
| IF | 12.084 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 47 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | BY20134 |