| RRC ID |
70403
|
| 著者 |
Ashraf MA, Akihiro T, Ito K, Kumagai S, Sugita R, Tanoi K, Rahman A.
|
| タイトル |
ATP binding cassette proteins ABCG37 and ABCG33 function as potassium-independent cesium uptake carriers in Arabidopsis roots.
|
| ジャーナル |
Mol Plant
|
| Abstract |
Radiocesium accumulated in the soil by nuclear accidents is a major environmental concern. The transport process of cesium (Cs+) is tightly linked to the indispensable plant nutrient potassium (K+) as they both belong to the group I alkali metals with similar chemical properties. Most of the transporters that had been characterized to date as Cs+ transporters are directly or indirectly linked to K+. Using a combinatorial approach of physiology, genetics, cell biology, and root uptake assay, here we identified two ATP-binding cassette (ABC) proteins, ABCG37 and ABCG33, as facilitators of Cs+ influx. A gain-of-function mutant of ABCG37 (abcg37-1) showed increased sensitivity to Cs+-induced root growth inhibition, while the double knockout mutant of ABCG33 and ABCG37 (abcg33-1abcg37-2) showed resistance, whereas the single loss-of-function mutants of ABCG33 and ABCG37 did not show any alteration in Cs+ response. In planta short-term radioactive Cs+-uptake assay along with growth and uptake assays in a heterologous system confirmed ABCG33 and ABCG37 as Cs+-uptake carriers. Potassium response and content were unaffected in the double-mutant background and yeast cells lacking potassium-uptake carriers transformed with ABCG33 and ABCG37 failed to grow in the absence of K+, confirming that Cs+ uptake by ABCG33 and ABCG37 is independent of K+. Collectively, this work identified two ABC proteins as new Cs+-influx carriers that act redundantly and independent of the K+-uptake pathway.
|
| 巻・号 |
14(4)
|
| ページ |
664-678
|
| 公開日 |
2021-4-5
|
| DOI |
10.1016/j.molp.2021.02.002
|
| PII |
S1674-2052(21)00048-4
|
| PMID |
33588076
|
| MeSH |
ATP Binding Cassette Transporter, Subfamily G / genetics
ATP Binding Cassette Transporter, Subfamily G / metabolism*
Arabidopsis / genetics
Arabidopsis / metabolism*
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Biological Transport
Cesium / metabolism*
Plant Roots / genetics
Plant Roots / metabolism*
Potassium / metabolism*
|
| IF |
12.084
|
| リソース情報 |
| 酵母 |
BY20134 |
