RRC ID 70455
Author Matsumoto S, Watanabe K, Imamura A, Tachibana M, Shimizu T, Watarai M.
Title Comparative Analysis Between Paramecium Strains with Different Syngens Using the RAPD Method.
Journal Microb Ecol
Abstract Paramecium spp. are a genus of free-living protists that live mainly in freshwater environments. They are ciliates with high motility and phagocytosis and have been used to analyze cell motility and as a host model for pathogens. Besides such biological characteristics, apart from the usual morphological and genetic classification of species, the existence of taxonomies (such as syngens) and mating types related to Paramecium's unique reproduction is known. In this study, we attempted to develop a simple method to identify Paramecium strains, which are difficult to distinguish morphologically, using random amplified polymorphic DNA (RAPD) analysis. Consequently, we can observe strain-specific band patterns. We also confirm that the presence of endosymbiotic Chlorella cells affects the band pattern of P. bursaria. Furthermore, the results of the RAPD analysis using several P. caudatum strains with different syngens show that it is possible to detect a band specific to a certain syngen. By improving the reaction conditions and random primers, based on the results of this study, RAPD analysis can be applied to the identification of Paramecium strains and their syngen confirmation tests.
Volume 84(2)
Pages 594-602
Published 2022-8-1
DOI 10.1007/s00248-021-01864-y
PII 10.1007/s00248-021-01864-y
PMID 34522990
MeSH Chlorella* Paramecium* / genetics Random Amplified Polymorphic DNA Technique Symbiosis
IF 3.356
Paramecium P. caudatum P. bursaria