RRC ID 70495
著者 Yadav M, Jalan M, Srivastava M.
タイトル Enhancer Dependent Repositioning of TCRb Locus with Respect to the Chromosome Territory.
ジャーナル J Mol Biol
Abstract Intranuclear position of several genes is dynamically altered during development concordant with their activation. To understand this dynamic, but non-random, nuclear organization, it is important to identify the relevant regulatory elements and trans acting factors. Murine TCRb locus gets activated during thymic development. Enhancer Eb is important for VDJ recombination at TCRb locus as it is critically required for establishment of recombination center. Our analysis revealed that TCRb locus gets located out of the chromosome territory specifically in developing thymocytes. Further, CRISPR/Cas9 based deletion mutagenesis established an unambiguous role of enhancer Eb in defining TCRb location relative to chromosome territory. The ability to reposition the target locus relative to chromosome territory highlights a novel aspect pertaining to activity of enhancers which may contribute to their ability to regulate gene expression. Additionally, our observations have implications for understanding the role of enhancers in three-dimensional genome organization and function.
巻・号 434(7)
ページ 167509
公開日 2022-4-15
DOI 10.1016/j.jmb.2022.167509
PII S0022-2836(22)00083-3
PMID 35202629
MeSH Animals Chromatin / metabolism Chromosomes / metabolism Enhancer Elements, Genetic* Genetic Loci* Mice Receptors, Antigen, T-Cell, alpha-beta* / genetics Thymocytes* / metabolism V(D)J Recombination*
IF 4.76
リソース情報
遺伝子材料 MSM Mouse BAC clone (RDB04214)