| RRC ID |
7051
|
| 著者 |
Cui HL, Li XY, Gao X, Xu XW, Zhou YG, Liu HC, Oren A, Zhou PJ.
|
| タイトル |
Halopelagius inordinatus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
Two extremely halophilic archaea, strains RO5-2(T) and RO5-14, were isolated from Rudong marine solar saltern in Jiangsu, China. Cells of the two strains were pleomorphic, motile and stained Gram-negative. Colonies were red-pigmented. Strains RO5-2(T) and RO5-14 were able to grow at 20-50 degrees C (optimum 37 degrees C), at 2.6-4.8 M NaCl (optimum 3.4-3.9 M NaCl), at 0.03-0.7 M MgCl(2) (optimum 0.5 M MgCl(2)) and at pH 5.5-8.0 (optimum pH 6.5-7.0). Cells lyse in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1). The 16S rRNA gene sequences of strains RO5-2(T) and RO5-14 showed 93.4-93.8 % similarity to the closest cultivated relative, Halosarcina pallida. The DNA G+C content of strains RO5-2(T) and RO5-14 was 61.0 mol% and 59.9 mol%, respectively. The DNA-DNA relatedness between strains RO5-2(T) and RO5-14 was 86.0 %. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains RO5-2(T) and RO5-14 represent a novel species in a new genus within the family Halobacteriaceae, for which the name Halopelagius inordinatus gen. nov., sp. nov. is proposed. The type strain is RO5-2(T) (=CGMCC 1.7739(T) =JCM 15773(T)).
|
| 巻・号 |
60(Pt 9)
|
| ページ |
2089-2093
|
| 公開日 |
2010-9-1
|
| DOI |
10.1099/ijs.0.018598-0
|
| PMID |
19854878
|
| MeSH |
DNA, Archaeal / genetics
DNA, Ribosomal / genetics
Fatty Acids / metabolism
Halobacteriaceae / classification*
Halobacteriaceae / genetics
Halobacteriaceae / isolation & purification*
Halobacteriaceae / metabolism
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S / genetics
Seawater / microbiology*
Sodium Chloride / metabolism*
|
| IF |
2.415
|
| 引用数 |
24
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 15773
JCM 14791
JCM 10706
JCM 14081 |
