論文 - 詳細
| RRC ID | 70520 |
|---|---|
| 著者 | Watanabe T, Nishio Y, Yamamoto Y, Shimizu T, Li XK, Okita H, Kuroda T. |
| タイトル | Photodynamic therapy with 5-aminolevulinic acid: A new diagnostic, therapeutic, and surgical aid for neuroblastoma. |
| ジャーナル | J Pediatr Surg |
| Abstract |
BACKGROUND:5-Aminolevulinic acid (ALA)-based photodynamic therapy (PDT) is widely used in cancer therapy because of the tumor-specific accumulation of photosensitizing protoporphyrin IX (PpIX). We aimed to assess the susceptibility of human neuroblastoma cell lines to ALA-PDT and determine the mechanism of PDT. METHODS:We used four human neuroblastoma cell lines (GOTO, NB9, IMR32, and NB1) and a gastric cancer cell line (MKN45) as a positive control. Cells were treated with increasing concentrations of ALA, and the ALA-induced production of PpIX in tumor cells was quantified using fluorescence spectrophotometry. PDT photocytotoxicity was measured by exposing the cells to a 630-nm irradiation for 10 min, and apoptotic cells stained with phosphatidylserine (PS) and propidium iodide (PI) were detected through flow cytometry. RESULTS:ALA cytotoxicity was not observed in any cell line. The intracellular concentration of PpIX increased in an ALA dose-dependent manner, and intracellular fluorescence of PpIX increased in a time-dependent manner. The viability of NB-1 cells treated with 250 μM 5-ALA rapidly decreased to 5%. Photocytotoxicity was observed in the following order: NB1, IMR32, NB-9, and GOTO. Photocytotoxicity was positively correlated with intracellular PpIX concentrations. PS+/PI- cells increased up to 21% after 12 h, and PS+/PI+ cells accounted for 35% of all cells after 24 h, which suggests that ALA-PDT induced apoptotic cell death. CONCLUSION:This study shows that neuroblastoma cell lines were susceptible to 5-ALA-PDT, resulting in persistent apoptotic cell death. LEVELS OF EVIDENCE:N/A for basic study. |
| 巻・号 | 57(7) |
| ページ | 1281-1285 |
| 公開日 | 2022-7-1 |
| DOI | 10.1016/j.jpedsurg.2022.02.028 |
| PII | S0022-3468(22)00201-9 |
| PMID | 35396087 |
| MeSH | Aminolevulinic Acid / pharmacology Aminolevulinic Acid / therapeutic use Cell Line, Tumor Humans Neuroblastoma* / drug therapy Photochemotherapy* / methods Photosensitizing Agents / pharmacology Photosensitizing Agents / therapeutic use |
| IF | 1.919 |
| リソース情報 | |
| ヒト・動物細胞 | MKN45(RCB1001) |