| RRC ID |
70521
|
| 著者 |
Yang YS, Ning SK, Lyu XH, Suo F, Jia GS, Li W, Du LL.
|
| タイトル |
Canavanine resistance mutation can1-1 in Schizosaccharomyces pombe is a missense mutation in the ubiquitin ligase adaptor gene any1.
|
| ジャーナル |
MicroPubl Biol
|
| Abstract |
In Schizosaccharomyces pombe, the can1-1 mutation confers resistance to the toxic arginine analog canavanine. This mutation has been assumed to disrupt a gene encoding an arginine transporter. In PomBase, the gene SPBC18H10.16 is currently designated can1. Here, we sequenced the genomes of three can1-1 strains. No mutations were found in SPBC18H10.16. Instead, these strains harbor an R175C mutation in the gene any1 (SPBC18H10.20c). any1 encodes an α-arrestin that acts as a ubiquitin ligase adaptor to downregulate plasma membrane amino acid transporters. Our findings indicate that can1-1 is not a loss-of-function mutation in an amino acid transporter gene, but a possible gain-of-function mutation in a gene encoding a negative regulator of amino acid transporters.
|
| 巻・号 |
2022
|
| 公開日 |
2022-1-1
|
| DOI |
10.17912/micropub.biology.000538
|
| PMID |
35300005
|
| PMC |
PMC8922049
|
| リソース情報 |
| 酵母 |
FY18665 |
